有机干扰物影响纳米Ag液灭活噬菌体MS2效果的实验研究

THE EXPERIMENTAL STUDY ON THE INACTIVATION EFFECT OF SILVER NANOPARTICLES AQUEOUS SOLUTION WITH ORGANIC INTERFERING SUBSTANCE TO BACTERIOPHAGE MS2

[目的]通过有机干扰物牛血清白蛋白(BSA)的存在与否比较研究纳米Ag液对噬菌体MS2的不同灭活效果,为纳米Ag在生活及环境中的实际应用提供基础资料。[方法]采用悬液定量灭活试验测定纳米Ag液对噬菌体MS2的灭活对数值(LIV)。[结果]在不加有机干扰物BSA时,100mg/L纳米Ag液作用60min,对噬菌体MS2的灭活对数值LIV为4.01log10,400mg/L作用20min,其LIV达4.12log10;而加入3%的BSA后,400mg/L纳米Ag液作用1440min(24h),对噬菌体MS2的LIV为3.61log10,800mg/L作用90min,其LIV为4.02log10。[结论]环境中有机干扰物的存在将会极大程度地降低纳米Ag液对噬菌体MS2的灭活效果。

[ Objective ] To investigate the inactivation efficacy of silver nanopartieles （Ag-NPs） aqueous solution to bacteriophage MS2 by adding organic interfering substance （BSA） or not, and thus to offer some basic information for the practical application of silver nanoparticles in life and environment. [ Methods] To determine the logarithm inactivation value （LIV） of Ag-NPs for bacteriophage MS2, suspension quantitative inactivation test were carried out. [ Results] While without BSA, MS2 was exposed to 100mg/L Ag-NPs for 60 minutes and 400mg/L for 20 minutes, the LIV to bacteriophage MS2 were 4.01log10 and 4.12log10, respectively; But with BSA （3%）, even MS2 was exposed to 400mg/L Ag-NPs for 1440min, the LIV was only 3.61log10, and to 800mg/L Ag-NPs for 90rain, LIV was 4.02log10. [Conclusion ] The existing of the organic interfering substance in environment could greatly cut down the inactivation effect of the solution of silver nanopartieles to bacteriophage MS2.