EMMPRIN和MT1-MMP在肝细胞肝癌中的表达及临床意义

Expressions of EMMPRIN and MT1-MMP in hapatocellular carcinoma and their significance

目的研究基质金属蛋白酶诱导因子(EMMPRIN)和模型金属蛋白酶1(MT1-MMP)在肝细胞肝癌中的表达及其与肝癌临床病理生物学行为之间的关系。方法采用免疫组化方法检测40例肝癌手术切除标本的EMMPRIN及MT1-MMP的表达情况。结果①MT1-MMP和EMMPRIN在肝癌组织中阳性表达率分别为62.5%和52.5%,明显高于在正常肝组织中的阳性表达率(P均<0.01);②EMMPRIN与肿瘤的转移、肝癌组织分化程度、肿瘤的大小有关;③MT1-MMP与肿瘤的转移、肿瘤的大小有关,与分化程度无关;④MT1-MMP蛋白与EMMPRIN蛋白在肝癌中的表达呈正相关(r=0.608,P<0.05)。结论MT1-MMP和EMMPRIN的表达均与肝癌的浸润转移有关,MT1-MMP和EMMPRIN的检测对于评估肝癌的预后有一定意义。

Objective To investigate the expression and clinical impact of membrane-type 1 matrix metallopreteinase （MT1-MMP） and extraceUular matrix metalloproteinase inducer （EMMPRIN） in hepatoeeUular carcinoma （HCC）. Methods Forty specimens obtained from HCC patientswere examined immunohistochemically using anti- EMMPRIN and anti- MT1-MMP monoelonal antibodies. Results （1）Tbe positive rates of EMMPRIN and MT1-MMP in HCC were much higher than those in the normal liver tissues（ P 〈0. 01 ）. （2）The positive rates and scorings of EMMPRIN were significantly lower in the cases of weU-ormiddle-differentiation,without-metastasis, and maximal dimeter of mass 〈 5 cm than that of poorly-differentiation, with-metastasis ,and maximal dimeter of mass ≥5 cm in the HCC tissues. （3）The expression of MT1-MMP in the HCC tissue was significantly correlated with the diameter of the number and the metastasis, but not correlated with the differentiation of tumor. （4）There was positive significant correlation between the expressions of MT1-MMP and EMMPRIN protein in HCC（ r =0.608 ,P 〈0.05 ）. Conclusions The expressions of MT1-MMP and EMMPRIN are related to the invasion and metastatic trend of HCC ,detecting the combined expression of them may give an objective indication on judging the biological behavior of the HCC.