幽门螺杆菌对GC及癌前病变抑癌基因和抑制细胞凋亡基因的影响

Effects of helicobacter pylori infection on expression of tumor suppression gene and regulatory gene of apoptosis in gastric cancer and precancerous lesions

目的探讨幽门螺杆菌(Hp)在胃癌(GC)和癌前病变中的作用及其与抑癌基因p53、抑制细胞凋亡基因Bcl-2表达的关系。方法选取胃镜活检标本107例,其中GC 28例,异型增生(DYS)14例,肠上皮化生(IM)16例,慢性萎缩性胃炎(CAG)34例,慢性浅表性胃炎(CSG)15例。尿素酶试验和Warthin-Starry银染色检测Hp,免疫组化SP法检测p53、Bcl-2基因蛋白的表达。结果Hp感染阳性组中GC和癌前病变的发生率高于Hp感染阴性组(P<0.05),p53、Bcl-2的阳性表达率在GC、DYS和IM中明显高于CSG(P均<0.05)。结论Hp与GC发生关系密切,持续Hp感染所致的慢性炎症可引起胃黏膜损伤,促进了p53基因的突变和Bcl-2基因的表达。

Objective To study the expressions of tumor suppression gene p53 and regulatory gene of apoptosis Bcl-2 in gastric mucosa lesions of gastric cancer patients with Helicobacter pylori infection. Methods Tissue samples from 107 patients underwent endoscopy were studied. Hp was examined with rapid urease test and Warthin-Starry staining, the expressions of p53 and Bcl-2 was detected by immunohistochemical assays. Results Disease rate of gastric cancer and precancerous lesion in Hp infection positive groups were higher than that in the Hp infection negative groups（ P 〈 0. 05 ） ;The expression rates of p53 and Bcl-2 in GC, DYS and IM were higher than in CSG （ P 〈 0. 05 ）. Conclusions There is the closely relation between Hp and gastric cancer. Hp last infection cause to chronic gastritis and damage stimulating expression of p53 and Bcl-2.