生芪降糖颗粒对2型糖尿病大鼠血糖、血脂及血管内皮素表达的影响

Effect of Sheng qi jiang tang ke li on blood glucose,lipid and endothelin-1 expression of the diabetic rats

目的探讨生芪降糖颗粒对糖尿病大鼠血糖、血脂及血管内皮素表达的影响。方法制作2型糖尿病大鼠模型,随机分为生芪降糖颗粒低剂量组、中剂量组、高剂量组、文迪雅组、对照组。正常组给予蒸馏水灌胃,模型组分别给予生芪降糖颗粒低剂量、中剂量、高剂量、文迪雅、蒸馏水灌胃,至血糖下降至正常组水平或与造模时比较有统计学差异为治疗有效。测定各组血糖、总胆固醇(TC)、甘油三酯(TG)、胰岛素、主动脉内皮素1(ET-1)的变化及ET-1mRNA表达。结果药物干预后生芪降糖颗粒中、高剂量组和文迪雅组血糖、TC、TG和胰岛素明显下降(P均<0.01)。文迪雅组和生芪降糖颗粒高剂量组ET-1和主动脉ET-1mRNA表达量较对照组均显著下降(P均<0.01)。结论①生芪降糖颗粒有抗糖尿病作用,并呈剂量依赖性;②生芪降糖颗粒降低ET-1mRNA的表达,可保护和改善糖尿病大鼠主动脉内皮和心肌的功能。

Objective To observe the effects of Sheng qi jiang tang ke li on blood glucose,lipid and endothelin- 1 （ ET- 1 ） expression in the diabetic rats. Methods The diabetic rat mode was made, and then experimental groups were randomizedly divided into Sheng qi jiang tang ke li low dose group, mid-dose group, high dose group, rosiglitazone group and control group. When the treatment was effective, the rats＇ plasma glucose were decreased to normal level or had significant difference compared with control model. The blood glucose, total cholesterin （ TC ）, triglyceride （ TG ）, insulin, ET-1, and ET- 1 mRNA were measured in different groups. Results After intervention of medicine, the blood glucose, TC,TG,insulin were decreased significantly in Sheng qi jiang tang ke li mid-dose group, high dose group and rosiglitazone group（ P 〈 0. 01 ）. The expressions of ET-1 protein and mRNA of vascular endo,theline were also decreased in the rosiglitazone group and high dose group of Sheng qi jiang tang ke li. Condusions （1）Sheng qi jiang tang ke li has effects on anti-diabedic mellitues, the effects present dosage dependence as well. （2）Sheng qi jiang tang ke li decreases ET-1 expression, and then protects vascluar endothline and cardiac muscle function.