东北地区少数民族基因组保存永生细胞库的建立

Establishment of Immortal lymphoblastoid Cell Lines for the Preservation of the Genomes of the Minority Nationalities in North Eastern China

作者采用ＥＢＶ转化，同时加入环孢霉素Ａ方法，对世居东北地区的鄂温克、鄂伦春和达斡尔３个少数民族建立了淋巴母细胞永生细胞库，共建系９１个。人类基因组多样性研究，是探讨各民族的起源、演进，研究基因突变与遗传病发生的重要组成部分。建立永生细胞库保存少数民族个体基因组，可为今后遗传多样性的研究提供长期的研究资料。

The immortal lymphoblastoid cell lines were established by EBV transformation of B cells and addition of cyclosporin A to inhibit the activity of T cells.In the present study,91 immortal cell lines of Ewenki,Oroqen and Dahur,the minority nationalities of North Easten China were established.This is an important part of the research of human genome diversity for the exploration of the origin and progression of different nationalities,and is also important to the research of gene mutation and the pathogenesis of the inherited diseases.