胰腺癌中Syk和VEGF-C的表达及其临床意义

Expression and clinical significance of syk and VEGF-C in pancreatic cancer

目的探讨脾酪氨酸激酶（Syk）和血管内皮生长因子（VEGF）-C在胰腺癌中的表达变化及其与淋巴转移的关系。方法应用免疫组化技术检测40例胰腺癌组织、正常胰腺组织和慢性胰腺炎组织中Syk和VEGF-C的表达情况。结果慢性胰腺炎、正常胰腺组织均表达Syk，其表达阳性率分别为77．8％、100％；在胰腺癌中多呈中等或弱阳性表达，阳性率为27．5％，显著低于前两者（P〈0．01）。Syk与肿瘤的发生部位、分化程度和淋巴结转移无关，而与TNM分期有关，Ⅰ～Ⅱ期者表达明显高于Ⅲ～Ⅳ期者（P〈0．05）。胰腺癌组织中VEGF—C阳性率为73％（29／40）。VEGF—C表达阳性者，淋巴结转移显著增多（P〈0．05）。VEGF—C与Syk的表达具有显著相关性（P=0．019）。结论Syk在胰腺癌中表达减低或缺失，与胰腺癌的发展显著相关，VEGF-C主要参与胰腺癌淋巴管生成的调控并促讲淋巴转移．在胰腺痛的侵袭和转移讨稗中Svk与VEGF—C且有协同作用．

Objective To investigate the expression of Syk and VEGF-C in pancreatic cancer and relation to lymphatic metastasis. Method The expression of Syk and VEGF-C was assayed by means of immunohistochemistry in 40 pancreatic carcinomas,9 chronic inflammation and 8normal tissues. Results The positive rates of Syk were 27.5% ,77. 8% and 100% ,respectively in pancreatic cancer, chronic inflammation and normal tissues. The expression of Syk in pancreatic cancer was significantly lower than that in other two kinds of tissues （ P 〈 0. 01 ）. There was no correlation between Syk expression and the site,differentiation and metastasis. Syk in Ⅰ -Ⅱ stages of pancreatic cancer showed stronger expression than that in Ⅲ-Ⅳ stages. The positive rate of VEGF-C was 73% in pancreatic cancer. The positive lymph node in positive VEGF-C group were higher than that in the negative group （P 〈 0. 05）. There was significant correlation between Syk and VEGF-C expression in pancreatic cancer （P = 0. 019）. Conclusions The expression of Syk in pancreatic cancer is significantly lower, which is related to development of pancreatic cancer. VEGF-C participats in the regulation of lymphangiogenisis,induces lymphangiogenisis in pancreatic cancer, and promotes the tumor lymphatic metastasis. Syk and VEGF-C have the effect of mutual cooperation in the process of pancreatic cancer.