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卵巢黏液性肿瘤中claudin-3、claudin-4的表达及其意义

The expression of claudin3 and claudin4 in ovarian mucous cystadenoma and it＇s significance
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摘要 目的 探讨claudin-3、-4在卵巢黏液性囊腺癌的发生、发展过程中的作用。方法应用免疫组织化学、原位杂交方法检测卵巢上皮、黏液性囊腺瘤、交界性黏液性囊腺瘤、黏液性囊腺癌的石蜡包埋组织中claudin-3、-4和claudin-4 mRNA的表达情况。结果 claudin-3、-4、claudin-4 mRNA在卵巢上皮及卵巢黏液性囊腺瘤中表达最弱；交界性黏液囊腺瘤中表达明显增强，却明显弱于黏液性囊腺癌（P〈0．05）；在低分化卵巢癌中的表达强于高分化癌及中分化癌（P〈0．05），中分化癌与高分化癌中的表达无统计学意义（P〉0．05）。结论 claudin-3、-4表达增强，与黏液性囊腺癌的发生、发展密切相关，与其分化程度有关，可能会成为诊断卵巢黏液性囊腺癌的特异性标记物。 Objective To explore the role of claudin-3, claudin-4 in carcinogenesis and progression of ovarian mucous cystoadenocarcinoma. Methods Immunohistochemical and in situ hybridization method were employed to detect the expression of claudin-3, -4, claudin-4 mRNA in ovary epithelium, mutinous cystadenoma, mutinous cystadenoma of borderline, mucous cystadenocarcinoma. Results claudin- 3, -4 expression was lowest in ovarian epithelium and mutinous cystadenoma, higher in borderline mutinous cystadenoma, and highest in ovarian cystadenocarcinoma（ P 〈 0.05 ）. The expression of claudin-3, -4 in poor differentiated ovarian carcinoma was stronger than that in well- differentiated and moderately differentiated carcinoma （ P 〈 0.05 ）, but there was no significant difference between well-differentiated and moderately differentiated carcinoma （P 〉0.05）. Conclusion The overexpression of claudin-3, -4 is correlated closely with the carcinogenesis and advancement of ovarian mutinous carcinoma, and may have correlation with the differentiated degree of ovarian carcinoma, claudin-3, -4 may become the specific markers of ovarian mucous cystadenocarcinoma.
作者 李传应 葛霞
机构地区 安徽省立医院病理科蚌埠医学院病理学教研室
出处 《国际肿瘤学杂志》 CAS 2008年第6期475-477,共3页 Journal of International Oncology
关键词 卵巢肿瘤 囊腺癌 黏液 原位杂交 Ovarian neoplasms Cystoadenocarcinoma, mutinous In situ hybridization
分类号 R737.31 [医药卫生—肿瘤]
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参考文献10

  • 1汤钊猷,朱世能,曹世龙.现代肿瘤学.第2版.上海:复旦大学出版社,2005:1242-1243.
  • 2Rangel LB, Agarwal R, D' Souza T, et al. Tight junction proteins claudin3 and claudin4 are frequently overexpressed in ovarian cancer but not in ovarian cystadenomas. J Clin Cancer Res, 2003, 9 (7) : 2567-2575.
  • 3Zhu Y, Brannstrom M, Janson PO, et al. Differences in expression patterns of the tight junction proteins, claudin 1 , 3, 4 and 5 in human ovarian surface epithelium as compared to epithelial in inclusion cysts and epithelial ovarian tumours. J Int Cancer, 2006, 118 ( 8 ) : 1884-1891.
  • 4Tsukahara M, Nagai H, Kamiakito T, et al. Distinct expression patterns of claudin-1 and claudin-4 in intraductal papillary-mucinous tumors of the pancreas. J Pathol Int, 2005, 55(2) : 63-69.
  • 5Cheung ST, Leung KL, Ip Yc, et al. Claudin-10 expression level is associated with recurrence of primary hepatocellular carcinoma. J Clin Cancer Res, 2005, 11 (21) : 551-556.
  • 6高志光,秦环龙.肠上皮细胞紧密连接的生物学功能及在肠屏障中的作用[J].肠外与肠内营养,2005,12(5):299-302. 被引量：33
  • 7Sakaguchi T, Kohler T, Gu X, et al. Shigells flexneri regulates tight junction-associated proteins in human intestinal epithelial ceils. J Cell Microbiol, 2002, 4(6): 367-381.
  • 8Hofman P. Pathological interactions of bacteria and toxins with the gastrointestinal epithelial tight junctions and/or the zonula adherens: an update. J Cell Mol Biol, 2003, 49( 1 ) : 65-75.
  • 9Andras IE, Pu H, Deli MA, et al. HIV-1 Tat protein alters tight junction protein expression and distribution in cultured brain endothelial cell. J Neurosci Res, 2003, 74(2) : 255-265.
  • 10Han X, Fink MP, Delude RL. Proinflammatory cytokines cause NO *-dependent and-independent changes in expression and localization of tight junction proteins in intestinal epithlial cells. Shock, 2003, 19(3) : 229-237.

二级参考文献24

  • 1Tsukita S, Furuse M, Itoh M, et al. Multifunctional strands in tight junctions [ J ]. Nat Rev Mol Cell Biol, 2001, 2 ( 4 ): 285-293.
  • 2Yeaman C, Grindstaff K K, Nelson W J, et al. New perspective on mechanisms involved in generating epithelial cell polarity [ J ].Physiol Rev ,1999,79( 1 ) :73-98.
  • 3Ebnet K, Aurrand-Lions M, Kuhn A,et al. The junctional adhesion molecule(JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: a possible role for JAMs in endothelial cell polarity[ J]. Journal of Cell Science ,2003,116(2) :3879-3891.
  • 4Furuse M,Hiiragi T,Fujimoto K,et al. Claudin-1 and -2:novel integral membrane proteins localizing at tight junctions with no sequence similarity to occluding [ J ]. J Cell Biol, 1998,141 (7):391-401.
  • 5Martin-Padura I, Lostaglio S, Schneemann M, et al. Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration [ J ]. J Cell Biol, 1998,142 ( 1 ): 117-127.
  • 6Tsukita S, Furuse M. Occludin and claudius in tight-junction strands: leading or supporting players? [J] Trends Cell Biol,1999,9(7 ) :268-273.
  • 7Laura L Mitic, Christina M, Van Itallie,et al. Molecular Physiology and Pathophysiology of Tight Junctions I. Tight junction structure and function: lessons from mutant animals and proteins[ J ]. Am J Physiol Gastrointest Liver Physiol, 2000, 279 ( 2 ):G250-G254.
  • 8Furuse M, Sasaki H, Fujimoto K, et al. A single gene product,claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts[ J]. J Cell Biol, 1998, 143 (2): 391-401.
  • 9Kubota K, Furuse M, Sasaki H,et al. Ca(2 + )-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions [ J ]. Curr Biol, 1999, 9(18) :1035-1038.
  • 10Citi S, Sabanay H, Jakes R,et al. Cingulin, a new peripheral component of tight junctions [ J ]. J Nature, 1988, 333 ( 6170 ):272-276.

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国际肿瘤学杂志
2008年 第6期

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