生长抑素(SS)对炎症痛大鼠免疫功能和电针作用的影响

Somatostatin(SS) Involved in Electro-Acupuncture and Immunity Regulation in AA Rats

目的:观察SS、电针腰夹脊穴对AA大鼠细胞免疫功能以及SS对电针作用的影响,探讨SS在针刺免疫调节作用的影响及可能的机制。方法:以AA大鼠为研究对象,应用流式细胞检测法、MTT法、放射免疫法等方法,观察SS和电针对大鼠CD4+、CD8+、IL-2、IL-6、TNF-α水平和脾淋巴细胞转化率等指标的影响。结果:鞘内注射SS和电针可以显著上调AA大鼠CD4+、CD8+、IL-2、LTT水平,下调IL-6、TNF-α的水平,电针可以显著上调AA大鼠脾淋巴细胞转化率。结论:SS是神经-内分泌-免疫网络中的重要介质,SS可能是针刺免疫调节中的一个重要调节点。

Objective： Studying the influence of somatostatin （SS） on FAC - induced hyperalgesia, acupuncture immunity, to explore the influence of SS on the effect of acupuncture regulation of immunity and their possible conjunct mechanism. Methods ：The study was designed to observe SS and influence of serum CD4^＋, CD8^＋, IL- 2, IL- 6, TNF- α, splenic lymphocyte transformation rate, by using methods of flowcytometry, MTT, radioimmunity, on FAC - induced hyperalgesia. Results ： Using intrathecal injection and electro - acupuncture, CD4^＋, CD8^＋ , IL - 2 be improved, IL - 6, TNF - α be decreased, LTT can be enhanced by electro - acupuncture. Conclusion ： SS may be an important regulative point on acupuncture regulation of immunity.