“肝心宁”对肝纤维化大鼠病理组织改变影响的实验研究

Experimental Research of Therapentic Effect of Ganxining Capsule on Expermental HF Rats

目的:观察"肝心宁"胶囊对实验大鼠肝纤维化的防治作用。方法:52只SD大鼠随机分为空白组、模型组、阳性对照组、中药低剂量组、中药高剂量组5组。采用改良复合因素法复制大鼠肝纤维化模型。空白组与模型组给予生理盐水10mL/kg灌胃,阳性对照组给予秋水仙碱0.125mg/kg治疗,中药高、低剂量组分别给予肝心宁26、6.5g/kg治疗。6周后,HE染色后比较各组大鼠肝脏病理组织改变。结果:在肝纤维化程度上各治疗组与模型组比较均有显著性差异(P<0.05或P<0.01),而以中药低剂量组最为显著(P<0.01);在改善肝脏炎症程度上中药高、低剂量组与模型组比较有显著性差异(P<0.05或P<0.01),而阳性对照组与模型组比较无显著性差异(P>0.05)。结论:"肝心宁"能有效预防实验大鼠肝纤维化形成。用中药防治肝纤维化,通过单纯增加药量来获取疗效的方法是不可取的。

Objective：To observe the preventive and therapeutic effect of C, anxining capsule on experimental HF rats. Methods：52 SD rats were randomly divided into 5 groups , the normal group,the model group,the positive control group, the high dose group, and the low dose group. HF rat model was duplicated with Improved Composite Factor Method. Rats of the normal group and the model group were administered normal sodium with dose 10mL/kg, positive control group were administered Colchicine with the dose 0. 125mg/kg. The high dose group and the low dose group were administered ：Ganxining with the dose 26,6. 5g/kg respectively. The liver pathology change of each group by HE coloration were compared 6 weeks later. Results：Compared with the model group ,each treated group has significant difference in the HF degree （P 〈 0. 05 or P 〈 0. 01 ） , the low dose group is the most significant difference in all groups （ P 〈 0. 01 ）. Compared with the model group,the high dose group and the low dose group has significant difference in hepar adiposum （P 〈 0.05 or P 〈 0. 01 ）, but the positive control group has not the significant difference （ P 〉 0. 05 ）. Conclusion ： C, an.xining capsule could effectively prevent HF of the experimental rats. It is inadvisable for Chinese medicine to treat HF by only increasing the drug quantity.