花生白藜芦醇合成酶基因家族序列克隆及分析

Cloning and analysis of resveratrol synthase gene family

以花生H2007为材料，从花生基因组中扩增出约500bp的白藜芦醇合成酶基因（Resveratrol synthase，RS）的保守序列，该序列与已发表的来源于花生的RS推导的蛋白序列相似性为91％。根据获得序列设计巢式基因特异性引物，以紫外辐射后花生叶片总RNA反转录成的cDNA作为模板，通过3’-RACE（Rapid Amplification of cDNA Ends），获得15个不同RS基因的片段，序列分析结果表明，扩增得到的15个白藜芦醇合成酶基因片段与已报道的基因的序列相似性为86％～98％。RS的部分编码区聚类分析结果表明这15个序列遗传距离在0．05之内（图5）。15个RS基因的3’-uTR碱基长度不等，最短的有69bp，最长的有244bp，两两比较相似性为23％～100％。四组RS基因的部分编码区聚类分析结果与3’-uTR区相同。

The conservative region of resveratrol synthase （RS） gene from peanut H2007 was amplified from genomic DNA, and the deduced amino acid shows 91% similarity with the RS sequence from peanut deposited in C.enebank. Nested PCR primers were designed to amplify the first strand cDNA transcribed form total RNA of ultra violet irradiated leaves, and 15 resveratrol synthase genes were obtained via 3＇-RACE （3＇- rapid amplification of c DNA ends） method. The homologys between RS gene family and published RS are 86%～98%. Phylogenetic tree based on resveratrol synthase （RS） in Arachis and other species indicated that the inherit distance is within 0.05. The length of 3＇ untranslated region （3＇- UTR） of 15 RS genes ranges from 69bp to 244bp. The homology between 3＇-UTR from different RS genes is 23% ～100%. Four pairs of RS genes are in the same group by phylogenetic tree based on translated region and 3 hntraslated region of RS.