属间杂交获得的甘蓝型油菜雄性不育材料的研究

Study of male sterile lines derived from intergeneric hybrids between Sinapis arvensis L. and Brassica napus L.

采用蕾期剥蕾授粉的方法,成功获得了新疆野生油菜野油2000-1和甘蓝型油菜Parol属间杂种。杂种F1育性极低,自由授粉条件下获得少量F2种子。在F2群体中成功选择到一株雄性不育株,用Parol做轮回亲本与不育株回交,BC1、BC2和BC3群体中可育株与不育株均呈1∶1分离。用波里马细胞质雄性不育的保持系和恢复系、681A的保持系和恢复系、隐性核不育86A的保持系及其它33个甘蓝型油菜品系与BC2群体中的不育株测交,测交后代可育株与不育株呈5∶3分离的组合有20个,呈1∶1分离的组合有14个,呈3∶1分离的组合有3个,有一个组合的后代完全可育,表明该雄性不育材料可能为显性核不育。

Intergeneric hybrids between Sinapis arvensis （Yeyou 2000 - 1 ）and Brassica napus var. Parol were produced by bud - pollination. The hybrids were amost sterile and only a few F2 seeds were harvested under the natural condition. A male sterile plant 200210 - 1 showing no pollen release upon flowering was selected from F2 population after open pollination. Using Parol as a recurrent parent to pollinate the male sterile plant, the ratio between male fertile and sterile plants was I： I in BC1 ,BC2 and BC3 generations. All except one F1 progenies from test crosses using restorer and maintainer lines of Polima and 681 CMS, maintainer line of recessive male genie sterility 86A and other 33 B. napus varieties or lines showed fertility segregation with the ratio of5：3 in 20 combinations, I： I inl4 combinations and 3： I in 3 combinations; and the progeny of one male sterile combination became fertile. All these results indicated that the male sterility of 200210 -1 most likely were dominant male genie sterility.