白癜风患者血清及皮肤组织液sICAM-1和IL-12的水平

Levels of Intercellular Adhesion Molecule-1and Interleukin-12 in Serum and Skin Tissue Fluid of Vitiligo Patients in Stable Stage

目的:探讨可溶性细胞间黏附分子-1(sICAM-1)和白细胞介素-12(IL-12)在稳定期白癜风患者血清及皮肤组织液中的变化。方法:将32例稳定期白癜风患者根据皮损分布分为寻常型与节段型,分别进行负压吸疱移植治疗,收集白斑及正常皮肤的疱液,同时抽取外周血5mL,用ELISA法检测皮肤组织液及血清中sICAM-1及IL-12的水平。结果:寻常型白癜风患者局部白斑处皮肤组织液sICAM-1及IL-12水平较非白斑处显著增高;而节段型白癜风白斑与非白斑处皮肤组织液sICAM-1及IL-12水平无显著性差异。稳定期白癜风患者血清sICAM-1及IL-12水平与对照组相比,均无显著性差异。结论:寻常型稳定期白癜风患者局部白斑皮肤微环境处于免疫异常状态,sICAM-1及IL-12可能参与了其发病的某一环节。

Objective To measure sICAM-1 and IL-12 between vitiliginous and non-vitiliginous patches in patients with stable vitiligo, and to compare them in serum with normal controls. Methods Thirty-two vitiligo patients in stable stage were divided into common type vitiligo and segmental type vitiligo. Suction blister fluid and peripheral blood were drawn and collected, and the enzyme-linked immunosorbent assay was used to determine the levels of sICAM-1 and IL-12 in serum and skin tissue fluid. Result The levels of sICAM-1 and IL-12 in skin tissue fluid were significantly higher in vitiliginous skin than that in non-vitiliginous patches in patients with common type vitiligo, whereas there was no significant difference between the two kinds of patches in patients with segmental type vitiligo. There were no significant difference in the serum levels of sICAM-1 and IL-12 between vitiligo patients in stable stage compared with those in normal controls. Conclusion The immune function is abnormal in vitiliginous skin of the common type vitiligo patients in stable stage. It is suggested that sICAM-1 and IL-12 be involved in the pathogenesis of vitiligo.