中国对虾幼虾荧光体内标记技术研究

Study on techniques of internal tagging by visible implant fluorescent elastomer in Fenneropenaeus chinensis

2004年7月,采用可视嵌入性荧光标记(Visible i mplant elastomer,VIE)对平均体长为4.75cm的中国对虾幼虾进行第6腹节的两部位标记,以研究VIE标记对中国对虾幼虾的适用性。10个家系的幼虾各取30尾,对其中的15尾进行标记,另15尾作为对照,共标记幼虾150尾。本研究分析了标记对虾和对照组的即时死亡率、生长和标记保持率。结果显示,标记对虾和对照组的即时死亡率低,前者为0.6%,后者无死亡。x2检验显示,二者不存在显著差异(P<0.05);通过对30、60d后体长的单因素方差分析,实验组和对照组在体长方面不存在显著差异(P<0.05),表明荧光标记对生长没有影响;标记后30、60d的标记保持率均很高,达到99%以上。2005年7月,对人工定向交尾建立的108个家系(平均体长为4cm)进行了大规模荧光标记,标记后30、60d的标记保持率均在99%以上,越冬后(210d后),对不同标记部位的标记保持情况进行了统计,标记保持率均在90%以上。

In July 2004,the suitability of visible implant fluorescent elastomer（VIE）for tagging juvenile shrimp Fenneropenaeus chinensis（Mean body length 4.75 cm）was investigated.Tags of five different colours were implanted at two body positions in the sixth abdominal segment.30 individuals in each of 10 families were selected randomly,among which 15 were VIE-tagged,while the remaining 15 were used as control.A total of 150 shrimps were tagged and their immediate mortality,growth and tag retention were assessed against the control over periods of 30 and 60 days.The immediate mortality of tagged shrimps was low（0.6%）and no animal died in the control,and x2 test revealed no significant difference between the tested and control groups（P〈0.05）.There was no significant difference in mean body length between tagged and untagged individuals over periods of 30 and 60 days,indicating that VIE tag had no negative effect on shrimp growth.Marker retention rate was over 99% through 30d and 60 d periods.In July 2005,108 families of F.chinensis（Mean body length 4cm）were tagged by VIE.Over periods of 30 and 60 days,tag retention was also more than 99%;and 210 days later,tag retention at different body positions was more than 90%.The successful application of VIE for marking the juvenile shrimps in this study is of significance for the breeding programme of F.chinensis.