罗氏沼虾大规模家系构建与培育技术研究

Study on large-scale family construction and rearing techniques for Macrobrachium rosenbergii

为建立罗氏沼虾育种项目,2006年利用罗氏沼虾3个群体,通过巢式交配设计,实现了大规模构建罗氏沼虾父系半同胞家系。实验中,每箱放置亲虾1雄5雌,按交配设计共布置100个交尾网箱;通过对培育条件及幼体数量的标准化,使每个家系在幼体培育的各个阶段的条件尽量保持一致,减少由于条件不一致造成的家系间的环境偏差。结果显示,500尾雌虾抱卵虾为271尾,亲虾抱卵率平均为54.2%,且每个网箱出现两尾以上抱卵虾的网箱数为92个,占全部网箱数的92%;通过标准化培育,最终建立了123个家系,其中含有父系半同胞家系37个。本试验结果为建立罗氏沼虾全同胞和半同胞家系提供了依据。

An experiment on large-scale family construction was done for Macrobrachium rosenbergii in 2006,in order to carry out selective breeding.Nested mating design was used for three M.rosenbergii groups to construct paternal half-sib families.A total of 100 net cages were used for the experiment,and five female and one male shrimps were put in each net cage.Rearing condition and larval density during each stage of development were standardized in order to reduce environmental effects on different families.271 among 500 female shrimps carried eggs,at an average egg-carrying percentage of 54.2%,and in each of 92 net cages（at 92% of the total number of cages）there were at least two egg-carrying females.123 families were obtained through standardized procedure for family construction,including 37 paternal half-sib families.The result of this study set up a foundation for constructing full-and half-sib families of M.rosenbergii.