摘要
从异叶银合欢K(164)和K(794)的根瘤中各分离到一株根瘤菌，并且，对K164根瘤菌的形态特征和生理生化特性进行了研究。K(164)根瘤菌在平板上培养2天，菌落呈圆形，灰白色，直径约1．2mm；革兰氏染色呈阴性，无芽孢，有美膜，两根极生鞭毛；杆菌大小为2．2×0．89μm，增代时间为1．69小时，属快生型；最适生长温度为30℃，在39℃持续2天不能存活，在20℃以下不能生长；不耐盐，较耐酸，在pH4.5～6．6范围内均能生长，但不耐碱；对庆大霉素和青霉素敏感；对供试的10种糖都能利用，不液化明胶，不利用淀粉，B．T．B试验未变色，不能进行3-酮基乳糖反应，硝酸盐反应呈阳性，石蕊牛奶试验能胨化、还原、产碱，牛肉膏蛋白胨培养生长差，能利用柠檬酸盐；在自生条件下具有吸H2酶活性；回接后结瘤率达100％，根瘤具有固氮酶和吸H2酶活性。
One fast-growing Rhizobium strain K164n was isolated from root nodules of Leucaena diversifolia. It was Gran-negative rod (2. 2×0.89μm) with subpolar flagella. Long doubling time (1. 69 hr) in YM liquid medium was observed. It grew well at 30℃ and did not grow both at 39℃ and in 0. 5% NaCl. The growth had a pH ranging from 4. 5 t0 6. 6. Physiological and biochemical characteristics of strain K164m were systematically studied. When inoculated, nodules was formed on the host plants. The nitrogenase activity was determined. Expression of H2 uptake activity of strain K164n in free-living was investigated.
基金
福建省科委资助
关键词
异叶银合欢
根瘤菌
特性
Leucaena diverstheia
Rhizobium
Characteristics
