血管紧张素Ⅱ对造血祖细胞优势扩增的影响

Effect of angiotensin Ⅱ on the advantaged differentiation and proliferation of haematopoietic progenitor

目的探讨血管紧张素Ⅱ(AngⅡ)及其受体拮抗剂氯沙坦对不同系别造血祖细胞分化的影响。方法悬浮培养的单个核细胞分为5组:促红细胞生成素(EPO)组(Ⅰ组),EPO+AngⅡ组(Ⅱ组),EPO+AngⅡ+氯沙坦组(Ⅲ组),AngⅡ组(Ⅳ组)及对照组(Ⅴ组)。巢式逆转录聚合酶链反应(nested-RT-PCR)检测血管紧张素Ⅱ1型受体(AT1R)基因的mRNA表达。计数红系爆式集落形成单位(BFU-E)和粒-单系集落形成单位(CFU-GM)。结果①EPO组及EPO+AngⅡ组于第6,9天可检测到AT1RmRNA表达;在相同时间点EPO+AngⅡ组较EPO组AT1R的mRNA表达量增多。②EPO+AngⅡ组较其余各组红系集落明显增多,粒系集落明显减少。AngⅡ组及对照组均未检测到红系集落。结论AngⅡ在EPO存在下与AT1受体结合促使红系祖细胞向红系优势分化。氯沙坦通过抑制AngⅡ与AT1受体结合,抑制红系分化。

Objective To investigate the effects of angiotensin Ⅱ （Ang Ⅱ ） and losartan on the advantaged differentiation of haernatopoietic progenitors. Methods Cultured mononuclear cells were divided into 5 groups： erythropoietin （EFO）group （group Ⅰ ） ,EPO ＋ Ang Ⅱ group（group Ⅱ ）, EPO ＋ Ang Ⅱ ＋ losartan group （ group Ⅲ ）, Ang Ⅱ group （ group Ⅳ ） and control group （group Ⅴ ）. The numbers of colony-forming unit-granulocyte-macrophage（CFU-GM）and burst-forming unit-erythroid（BFU-E）colonies were counted. Nested RT-PCR was used to determine the expression of angiotensin Ⅱ type 1 receptor（ATiR）. Results ①ATIR mRNA was found at day 6 and 9 in EPO group and EPO ＋ Ang Ⅱ group. The expression of ATIR rnRNA in EPO ＋ Ang Ⅱ group was higher than in EPO group. ②Compared with the other groups, the numbers of BFU-E colonies in EPO ＋ Ang Ⅱ group increased significantly while the numbers of CFU-GM decreased significantly. The BFU-E colonies were not found in Ang Ⅱ group and control group. Condusion Angiotensin Ⅱ has stimulatory effect on erythroid progenitors with erythropoietin by binding with AT1 receptor. Through blocking the binding of angiotensin Ⅱ and ATIR, losartan inhibits the proliferation of erythroid progenitors.