芹菜素对急性局灶性大鼠脑缺血/再灌注损伤的神经保护作用

Neuroprotective Effects of Apigenin on Acute Transient Focal Cerebral Ischemia-reperfusion Injury in Rats

目的:探讨芹菜素对急性局灶性大鼠脑缺血/再灌注损伤的神经保护作用。方法:采用改良线栓法建立局灶性脑缺血/再灌注模型,对大鼠进行神经行为学评分、脑组织TTC染色及电镜观察,测定脑含水量和EB含量。结果:模型组和芹菜素组出现不同程度的神经行为学评分异常;TTC染色示模型组出现白色梗死灶;电镜下4h模型组海马CA1区和缺血区皮层的胶质细胞内和毛细血管周围水肿明显,神经元出现核固缩,而芹菜素组病变明显减轻;芹菜素组脑含水量和EB含量较模型组各对应时点明显减少。结论:芹菜素对急性缺血/再灌注脑损伤有一定的保护作用。

Objective ： To evaluate the neuroprotective effects of apigenin on acute transient focal cerebral ischemia-reperfusion injury in rats. Methods ： The acute transient focal cerebral ischemia-reperfusion model was established with modified method of insertion of thread fish nylon into and staying for two hours and then withdrawing from middle cerebral artery in rats. In experiment groups the neurological behavior scores, TTC stain of brain slices, neurocyte morphology were observed, and brain water content and Evans blue （EB） content were measured. Results ： Abnormal neurological behavior scores were existed in apigenin-treated group and model group. Typical cortical infarct lesions in model group were found by TTC stain. The neurocyte morphology in model group 4 hours was found in swollen glis and obvious edema near capillary and within nervous process, and karyopycnosis in neuron of ischemic cortex and hippocampus CA1 under electric microscope. However lesion was alleviated in apigenin-treated group. The brain water content and EB content in apigenin-treated group were lower than model group. Conclusion ： Apigenin may play an important neuroprotective role in acute transient focal cerebral ischemia-reperfusion injury in rats.