红芪总多糖对大鼠肺纤维化及其转化生长因子β1-干预的实验观察

An Experimental Observation of Pulmonary Fibrosis in Rats and Its Transforming Growth Factor β_1 Treated by Total Hedysarum Polybotyssaccharide

目的:提供肺纤维化治疗方案及研究其机理。方法:120只Wistar大鼠,随机抽出24只作为正常组,气管内注入生理盐水(作为阴性对照),剩余96只经气管内注入博莱霉素造模成功后随机分为模型组24只(作为阳性对照),强地松组24只,红芪总多糖组24只,红芪总多糖合小剂量强地松组24只,分别给予相应药物治疗。以上各组动物在第7、14、30、60 d每组随机抽出6只处死进行病理切片观察,电子计算机图像分析仪进行组织形态学、胶原和转化生长因子β1定量分析。结果:红芪总多糖合小剂量强地松组对模型动物干预最强。结论:红芪总多糖合小剂量强地松联合应用治疗大鼠肺纤维化优于经典方法,其机制可能与红芪总多糖抑制转化生长因子β1有关。

Objective： To treat pulmonary fibrosis and study its mechanisms. Methods： Choosing randomly 24 Wistar rats with normal sodium intratracheal injection as normal control group, the rests with heomycin A,5 induced fibrosis were divided randomly into model group 24 as positive control, prednisone group 24, total hedysarum polybotyssaccharide（THPS） group 24, THPS and small dose prednisone group 24, and treated with different drugs. 6 rats of every group were put to death and observed pathological section, using imaging processing computer to quantitative analysis histomorphology, collagen, and transforming growth β1 （TGF-β1 ） on 7,14,30,60 days. Results： The group treated by THPS combination small dose prednisone showed up the most effects in the 3 treatment groups. Conclusion ： THPS combination small dose prednisone to treat pulmonary fibrosis of rats is better than classic ways and its efficacy of inhibition TGF-β1 may be a mechanism.