丹参酮ⅡA对Hela细胞增殖和细胞周期的影响

Effects of Tanshinone Ⅱ A on hela cell proliferation and cycling

目的通过体外细胞培养的方法,研究丹参酮ⅡA(TanⅡA)对Hela细胞增殖和细胞周期的影响,并与甲氨蝶呤(MTX)比较,探讨TanⅡA在类风湿关节炎治疗方面的潜在价值。方法用MTT法计算不同浓度的TanⅡA及MTX处理后细胞生长抑制率(IR%);用碘化丙啶(PI)染色流式细胞术(FCM)检测不同浓度TanⅡA作用及MTX作用下细胞周期变化。结果TanⅡA、MTX均可对体外培养的Hela细胞产生增殖抑制作用,且均有剂量依赖关系。MTX的IC50值小于TanⅡA,而TanⅡA的最大抑制率约为MTX的4倍。TanⅡA、MTX均可使Hela细胞细胞周期的构成发生明显的变化,使S期细胞减少、G0/G1期细胞增多。结论TanⅡA、MTX均可对体外培养的Hela细胞产生增殖抑制作用,且均表现出剂量依赖关系。TanⅡA、MTX均可对Hela细胞细胞周期的构成产生影响,使S期细胞减少、G0/G1期细胞增加。通过以上实验提示TanⅡA在抑制细胞增殖方面类似于MTX在治疗RA方面有潜在价值。

Objective To investigate the effects of Tanshinone ⅡA （Tan ⅡA） on proliferation and cycling of Henrietta Lacks strain of cancer cells （Hela cells） as compared with methotrexate （MTX）, and to identify the potential of Tan ⅡA for treatment of rheumatoid arthritis （RA）. Methods We used the MTT method to measure the inhibition rate （IR%） and flow cytometry to detect the changes in cell cycle of Hela cells treated with varied levels of Tan ⅡA or MTX. Results Tan ⅡA and MTX were shown to inhibit the growth of Hela cells in a dose-dependent manner. The value of IC50 of MTX was lower than of Tan ⅡA, and the maximum inhibition rate of Tan ⅡA was four-fold higher than that of MTX. Both Tan ⅡA and MTX had significant effects on cell cycle of Hela cells along with increasing doses （P〈0.05）, resulting in fewer S-phase and more G0/G1-phase cells. Conclusion Tan ⅡA and MTX showed dose-dependent inhibition of Hela cell proliferation. The two agents also resulted in fewer cells at S-phase and more at G0/G1-phase. These findings suggested the MTX-like therapeutic potential of Tan ⅡA in RA.