替普瑞酮诱导阿尔茨海默病模型大鼠海马热休克蛋白70表达及对其学习记忆能力的影响

Geranylgeranylacetone induce the expression of heat shock protein 70 expression in hippocampus of Alzheimer＇s model rats and its effect on learning and memory ability in the rats

目的双侧海马注射Aβ1-42建立阿尔茨海默病大鼠模型，观察替普瑞酮诱导大鼠海马热休克蛋白70表达及对其行为学的影响。方法72只SD大鼠随机分为替普瑞酮组、模型组与对照组，双侧海马注射Aβ1-42建立阿尔茨海默病大鼠模型，对照组海马注射等量生理盐水。替普瑞酮组予替普瑞酮800mg／kg／d灌胃，余两组予等量生理盐水灌胃。术后1、7、14和21d并于Y迷宫行为学测试后处死大鼠。采用RT—PCR和western—blot方法，观察各组大鼠海马HSP70表达的变化。结果术后14和21d模型组学习记忆能力较对照组明显减退（P〈0．01），而替普瑞酮组大鼠较模型组有所改善（P〈0．01）。术后7、14和21d模型组大鼠海马HSP70表达较对照组逐渐减少（P〈0．05），替普瑞酮组HSP70表达明显增加（P〈0.05）。结论替普瑞酮能够诱导AD模型大鼠海马HSP70表达，从而减轻神经元损害，改善大鼠的学习记忆能力。

Objective To study that Geranylgeranylacetone （GGA） induce the expression of heat shock protein 70 expression in hippocampus of Alzheimer＇s model rats and its effect on learning and memory ability in the model rats induced by Aβ1-42 injection into hippocampus. Method 72 health SD rats were randomly divided into GGA group, model group was injected with Aβ1-42 and control group was injected with normal saline into hippocampus. Y maze test was used to detect the learning and memory ability of the rats at the 7th, 14th and 21 st day after injection into hippocampus separately. HSP70 expression in hippocampus tissues were detected by RT-PCR and western-blot as soon as Y maze test has been finished. Result The learning and memory ability of the rats in model group decreased significantly at the 14th and 21st day after injection than those in control group （ P 〈0. 05）, but not too much changed in GGA group （ P 〈0. 05）. HSP70 expressions in hippocampus tissues in model group decreased gradually after injection Aβ1-42, but increased in GGA group at the 7th, 14th and 21 st day after injection. Conclusion GGA can induce the expression of HSP70 in hippocampus of Alzheimer＇s model rats and ameliorate the neuron impairment as well as the learning and memory ability of the rats.