丹参注射液联合地塞米松对哮喘大鼠CD4^＋ CD25^＋调节性T细胞的影响

Influence of danshen injection combined with dexamethasone on CD4^＋ CD25^＋ regulatory T cells of asthmatic rats

目的探讨丹参注射液联合地塞米松（DXM）抑制哮喘气道炎症的免疫学机制。方法50只Wistar大鼠随机分成正常对照（NC）组、哮喘组、丹参组、DXM组、联合用药组，计数支气管肺泡灌洗液（BALF）中细胞总数并分类，HE染色行肺组织病理学检查，流式细胞仪检测外周血单个核细胞（PBMCs）中CD4^＋CD25^＋调节性T细胞（CD4^＋CD25^＋Treg）比例，ELISA检测BALF中IL-4、IL-5含量。结果与哮喘组比较，药物干预组细胞总数、中性粒细胞（Neu）、淋巴细胞（Lym）、嗜酸性粒细胞（Eos）百分率下降（P〈0．05，P〈0．01），联合用药组下降程度大于丹参组、DXM组（P〈0．05）。哮喘组呈显著炎症变化，丹参组呈中度炎症变化，DXM组呈轻度炎症变化，联合用药组无炎症改变。与哮喘组比较，药物干预组CD4^＋CD25^＋ Tres／CD4^＋ T升高（P〈0．05），IL-4、IL-5含量下降（P〈0．05），联合用药组CD4^＋CD25^＋ Tres／CD4^＋ T升高程度和IL-4、IL-5下降程度大于丹参组和DXM组（P〈0．05）。结论丹参注射液具有抑制哮喘大鼠气道炎症的作用，和DXM联合应用后，抑制作用更加明显，该作用可能和促进CD4^＋CD25^＋ Treg产生，进而抑制IL-4、IL-5产生，纠正Th1／Th2失衡，最终减轻气道炎症有关。

Objective To investigate the immunological mechanism of inhibitory effect of Danshen injection combined with dexamethasone （DXM） on asthmatic airway inflammation. Methods 50 Wistar rats were randomly divided into normal control （NC）, asthma, Danshen, DXM and Danshen ＋ DXM group. Cytology study of Bronchoalveolar lavage fluid （BALF） was conducted. Pathology of lung tissue was done through HE. Flow cytometry was used to detect CD4 ^＋ CD25^＋ regulatory T Cells （CD4^＋ CD25^ ＋ Treg） ratio in peripheral blood mononuclear cells （PBMCs）. IL-4 and IL-5 levels in BALF were detected by ELISA. Results Total cells number, percentage of lymphocytes, neutrophils and eosinophils （Eos） in BALF of the three treated groups were lower than that in asthma group （ P 〈 0.05, P 〈 0.01 ）, particularly in Danshen ＋ DXM group, which showed significant difference as compared with the other two treated groups （ P 〈 0.05 ）. There was severe inflammation in lung tissue of asthma group, moderate inflammation in Danshen group and DXM group, and no inflammation of Danshen ＋ DXM group. CD4^＋ CD25^＋ Treg/CD4^＋ T ratio in the three treated groups were higher than that in asthma group, and the levels of IL-4 and IL-5 were lower than those in asthma group （ P 〈 0. 05）. In Danshen ＋ DXM group, it showed significant difference on the change of CD4^＋ CD25^＋ Treg, IL-4 and IL-5 as compared with other treated groups （ P 〈 0.05 ）. Conclision Danshen injection combined with DXM could suppress airway inflammation in asthmatic rats, which may be through increasing the expression of CD4^＋ CD25^＋ Treg, decreasing the levels of IL-4 and IL-5 and resuming the balance of Th1/Th2.