γ射线辐照对β-淀粉样肽诱导红细胞膜损害的作用

Effect of gamma ray irradiation on β-amyloid peptide-induced damage of erythrocyte membrane

目的:研究γ射线辐照对β-淀粉样肽(Aβ)诱导的红细胞膜损害的保护作用.方法:红细胞经50 Gy和100 Gyγ射线照射后,再用Aβ处理,分别用分光光度法测定红细胞溶血率和渗透脆性;扫描电镜观察细胞形态;1,6-二苯基-1,3,5-己三烯(DPH)荧光探针标记检测细胞膜流动性.结果:γ射线辐照可抑制红细胞的自然溶血和Aβ导致的溶血,但只有100 Gy辐照的差异具有统计学意义(P<0.05),使自然溶血率和Aβ引起的溶血率由(3.76±0.50)%,(11.90±1.50)%分别降至(1.87±0.09)%和(7.90±0.90)%.50 Gy和100 Gy的辐照均可降低Aβ引起的细胞变形程度,增加细胞膜的流动性(P<0.05),荧光探针的各相异性值由0.36±0.01分别降至0.33±0.00和0.32±0.01,并提高红细胞对低渗溶液的耐受性,50%溶血时的NaCl浓度由82.3 mmol/L分别降至80.7 mmol/L和76.9 mmol/L.结论:在本实验条件下,γ射线辐照可降低Aβ对红细胞膜的损伤,并可增加红细胞膜的流动性及稳定性.

AIM： To investigate the effect of gamma-ray irradiation on β-amyloid peptide-induced damage of erythrocyte membranes. METHODS： Erythrocytes were irradiated with gammaray （50 and 100 Gy ） before treated with β-amyloid peptide （Aβ）. Hemolysis rate, osmotic fragility, morphology and membrane fluidity of erythrocyte were determined by means of spectroscopy, scan electron microscopy and fluorescent probe, respectively. RESULTS： Gamma-ray irradiation inhibited erythrocyte hemolysis in a dose-dependent manner, although only the 100- Gy irradiation had decreased the rates of Aβ-induced and spontaneous hemolysis from （ 3. 76 ± 0.50 ） % and （ 11.90 ± 1.50 ） % to （1.87±0.09）% and （7. 90 ± 0.90）%, respectively, showing significant inhibition （ P 〈 0.05 ）, Treatment of erythrocytes with both gamma-ray doses decreased morphological alteration and anisotropy values of the probe in membrane of the cells in the presence of Aβ（P 〈0.05） , from 0.36 ±0, 01 to 0.33 ± 0, 00 and 0.32 ±0.01, respectively. The osmotic fragility of erythrocytes, expressed as the NaC1 concentrations of the buffer that corresponding to 50% cell lysis, decreased from 82. 3 mmol/L （control） to 80.7 mmol/L（50 Gy） and 76.9 mmol/L （ 100 Gy） , respectively. CONCLUSION： Gamma-ray irradiation protected erythrocyte membranes from damages induced by β- amyloid peptide. This can be attributed to that gamma irradiation increases membrane fluidity and stability of the cells.