摘要
目的:研究单侧输尿管梗阻(UUO)大鼠肾组织p27的表达,同时观察TGF-β mRNA在各组的变化,探讨UUO模型中TGF-β与p27的关系。方法:60只SD大鼠随机分成假手术组(SOR)、UUO模型组。于术后第7、14、21天分别处死各组大鼠10只。用HE染色动态观察肾脏病理变化,免疫组织化学法测定p27的蛋白表达及动态变化,RT-PCR法测定p27 mRNA和TGF-β mRNA的水平。结果:SOR组肾小管上皮细胞p27蛋白及肾皮质p27 mRNA的表达均强,UUO组随着间质纤维化程度的加重,p27的表达逐渐减弱,而TGF-β mRNA随着肾间质纤维化程度的加重,其表达呈上升趋势;UUO组TGF-β mRNA与p27 mRNA成负相关。结论:p27参与了UUO大鼠肾间质纤维化的发病过程;UUO大鼠肾间质纤维化模型中TGF-β促纤维化机制可能与p27的下调相关。
AIM： To explore the expression of p27 and the changes of TGF-β in the renal tubule on the process of renal interstitial fibrosis caused by unilateral ureteral obstruction（UUO） in rats and to investigate the relationship between TGF-β and p27 in renal interstitial fibrosis rats.METHODS： Sixty rats were randomly divided into sham-operated group（SOR） and UUO group.Ten rats from each group were sacrificed on days 7,14 and 21 after operation respectively.The renal pathological changes were dynamically observed by HE.The expression and dynamic changes of p27 were detected by immunohistochemistry.The levels of p27 mRNA and TGF-β mRNA were detected by RT-PCR.RESULTS： The expression of p27 in renal tubular epithelial cells and p27mRNA was strongly positive in SOR group.With the aggravation of interstitial fibrosis,the expression of p27 gradually decreased in UUO group while the expression of TGF-β increased.There was a negative correlation between TGF-β and p27.CONCLUSION： P27 is involved in the fibrosis of renal mesenchyme in rats with UUO.The mechanism of TGF-β accelerating fibrosis may relate to the reduction of p27 expression in UUO rats.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2008年第8期808-810,813,共4页
Chinese Journal of Cellular and Molecular Immunology
