用新型汉坦病毒通用引物检测HFRS患者血清中病毒核酸

Detection of Hantavirus Genome by New Pairs of Hantavirus Universal Primers in HFRS Patients' Serum

目的:提高汉坦病毒(HV)的检出率。方法:设计了两对新的汉坦病毒(HV)通用引物,建立新的RT-PCR方法,对流行于湖北省不同地区的肾综合征出血热(HFRS)患者血清进行了检测。结果:在对166份血清中HV RNA的检测显示HTN型阳性率为80.1%(133/166),SEO型为19.9%(33/166);对不同浓度的HV RNA检测结果显示,本法最低可检测出血清中72 pg病毒基因组。结论:说明本法用于检测HFRS患者血清中HV RNA的特异性和敏感性均很高,且我国中部地区HFRS流行以HTN为优势型,这一结果不仅为HFRS的诊断提供了新的手段,也为HFRS的防治提供了新的依据。

Objective： To improve the detection rate of Hantavirus. Methods： Two new pairs of Hantavirus universal primers was designed to detect the Hantavirus genome RNA in hemorrhagic fever with renal syndrome（HFRS） patient＇s serum in Hubei Province by RT-PCR. Results： In the 166 serum, 80.1% （133/166） were detected to be HTN and 19.9% （33/166） were SEO in serology typing. At the same time, even a low level of Hantavirus genome RNA （≥72 pg） could be detected by this method. Conclusion： It is an excellent method to detect Hantavirus genome in HFRS patients serum with both high specificity and sensitivity, and HTN is a dominant Hantavirus serotype in Hubei province.