臭氧溶液对HBV体外感染的HepG2细胞的抗病毒作用

Anti-Virus Effects of Ozone Solution on Human HepG2 Cell Line Infected with HBV in Vitro

目的:建立乙型肝炎病毒(HBV)体外感染人肝癌HepG2细胞的实验模型,并研究臭氧溶液对感染了HBV后的HepG2细胞的抗病毒作用。方法:乙肝阳性血清与HepG2细胞共孵育建立体外感染模型,采用倒置相差显微镜检测细胞形态,MTT法检测细胞成活率,ELISA法检测HBsAg和HBeAg表达水平,HBV荧光定量PCR检测HBV DNA表达量。结果:体外感染了HBV DNA的HepG2细胞能持续稳定分泌HBsAg和HBeAg,并能产生HBV DNA,通过低浓度臭氧溶液作用后,细胞形态无明显差异,细胞成活率无明显影响(P>0.05),HBsAg和HBeAg均转阴(P<0.01),HBV DNA拷贝量亦明显降低(P<0.01)。结论:较低浓度臭氧溶液对感染了HBV的HepG2细胞有明显的抗病毒作用,对细胞并无损伤。

Objective： To establish a culture system of HBV positive serum infected HepG2 cells in vitro,and to study the anti-virus effect of ozone solution on HepG2 cells infected with HBV.Methods： The hepatitis B masculine blood serum and the HepG2 cells altogether were fostered to establish the infection cell model in vitro.Cellular morphological change was observed by phase contrast microscope,cell viability was evaluated by MTT assay,HBsAg and HBeAg in cell culture medium were detected by ELISA assay kit and HBV-DNA copies by FQ-PCR.Results： HepG2 cells infected with HBV in vitro could product HBeAg and HBsAg stably and duplicate HBV DNA continuously.Under low concentration of ozone solution,no significant difference in cell morphology and cell survival rate was found when compared with that in the control group（both P〉0.05）,but the expression of HBeAg and HBsAg reduced（P〈0.01） and HBV DNA copies decreased significantly（P〈0.01）.Conclusion： Low concentration of ozone solution had obvious anti-virus effect on the HepG2 cells infected with the HBV in vitro.