白藜芦醇对人胃癌MGC803细胞形态影响和相关增生因素的变化

Effects of Resveratrol on Cell Morphology and Related Factors of Gastric Cancer Cell in vitro

目的研究白藜芦醇对人胃癌MGC803细胞形态的影响和相关增生因素的变化。方法用MTT法测定药物敏感性;光镜、电镜、MTT法活细胞计数、流式细胞仪观察其生物学特征。结果0.1g/L、0.2g/L白藜芦醇可以抑制MGC803细胞于S期,白藜芦醇能抑制MGC803细胞生长增生,并呈浓度依赖性。结论0.1g/L、0.2g/L白藜芦醇能明显地抑制MGC803细胞增生。

Objective To study the effects of resveratrol on cell morphology and related factors of gastric cancer cell line in vitro. Methods Drug sensitivity was detected by MTT assay . Changes of its biological characteristics were determined using light microscopy , electron microscopy cell counting by MTT assay ,flow cytometry（FCM） . Results Resveratrol （0.1 g/L,0.2g/L） could arrest the sus- pended gastric cancer cell （MGC803） to S phase respectively. Resveratrol significantly inhibited growth and proliferation of MGC803 cells in dosedependent manner. Conclusion Resveratrol （0. 1g/L ,0.2g/L ） may inhibit M GC803 cell growth.