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玻璃化冷冻技术在保存人类不同发育阶段卵细胞和胚胎中的应用 被引量：9

Application of Vitrification of Human Oocytes and Embryos at Different Developmental Stages
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摘要 玻璃化冷冻技术已逐渐成为辅助生育技术中重要的应用技术之一,在保存未成熟卵细胞、成熟卵细胞、原核期胚胎、卵裂早期胚胎及囊胚方面均取得成功,并相继有临床妊娠分娩的报道。本文总结了玻璃化冷冻技术在保存人类不同发育阶段卵细胞和胚胎方面的应用。 Vitrification of human oocytes and embryos has become an important assisted reproductive technology. It can be used to cryopreserve immature oocytes, mature oocytes, pronuclear embryos, blastomeres, and blastocysts. This article reviewed the clinical application of vitrification of human oocytes and embryos at different developmental stages.
作者 丁颖 邓成艳
机构地区 中国医学科学院北京协和医学院北京协和医院妇产科
出处 《中国医学科学院学报》 CAS CSCD 北大核心 2008年第3期348-353,共6页 Acta Academiae Medicinae Sinicae
关键词 玻璃化冷冻 卵细胞 胚胎 辅助生育技术 vitrification oocytes embryos assisted reproductive technology
分类号 R711.6 [医药卫生—妇产科学]
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参考文献30

  • 1Liebermann J, Nawroth F, Isachenko V, et al. Potential importance of vitrification in reproductive medicine [ J ]. Biol Reprod, 2002, 67 (6) : 1671-1680.
  • 2Boiso I, Marti M, Santalo J, et al. A confocal microscopy analysis of the spindle and chromosome configurations of human oocytes cryopreserved at the germinal vesicle and metaphase Ⅱ stage [ J ]. Hum Reprod, 2002, 17 ( 7 ) : 1885-1891.
  • 3Oktay K, Newton H, Aubard Y, et al. Cryopreservation of immature human oocytes and ovarian tissue: an emerging technology [ J ]. Fertil steril, 1998, 69 ( 1 ) : 1-7.
  • 4Cha K, Chung HM, Lim JM, et al. Freezing immature oocytes [J]. Mol Cell Endocrinol, 2000, 169(12) :43-47.
  • 5Wu J, Zhang L, Wang X. In vitro maturation, fertilization and embryo development after ultrarapid freezing of immature human oocytes [ J ]. Reproduction, 2001, 121 ( 3 ) : 389-393.
  • 6Isachemko V, Montag M, Isachenko E, et al. Aseptic vitrification of human germinal vesicle oocytes using dimethyl sulfoxids as a cryoprotectant [ J ]. Fertil Stefil, 2006, 85 (3) :741-747.
  • 7Tucker M J, Morton PC, Wright G, et al. Clinical application of human egg cryopreservation [ J ]. Hum Reprod, 1998, 13(11) :3156-3159.
  • 8Lucena E, Bernal DP, Lucena C, et al. Successful ongoing pregnancies after vitrification of oocytes [ J ]. Fertil Stefil, 2006, 85(1) :108-111.
  • 9Chen SU, Lien YR, Chao Kh. Cryopresevation of mature human oocytes by vitrification with ethylene glycol in straws [J]. Fertil steril, 2000, 74(4) :804-808.
  • 10Selman H, Anqelini A, Bemocchi N. Ongoing pregnancies after vitrification of human oocytes using a combined solution of ethylene glycol and dimethyl sulfoxide [ J ]. Fertil Steril, 2006, 86(4) :997-1000.

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中国医学科学院学报
2008年 第3期

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