顺铂水针剂与粉针剂体外细胞毒作用的差异性研究

The comparison of the cytotoxicity between cisplatin solution and cisplatin powder in vitro

目的:比较两种不同剂型顺铂针剂的细胞毒效应。方法:用含不同终浓度顺铂水针剂与粉针剂的培养液培养Hela细胞系,采用四甲基偶氮唑蓝比色法(MTT)检测细胞存活率并计算顺铂的50%抑制浓度(IC50),分析比较两种不同顺铂剂型针剂在体外的细胞毒效应。结果:相对于顺铂粉针剂,相同终浓度顺铂水针剂作用的Hela细胞存活率较低,其IC50值分别为(14.01±0.07)μg·ml-1及(15.03±0.26)μg·ml-1(P=0.003)。其中在(0.1、1、10)μg.ml-1组中细胞存活率存在差异。结论:顺铂水针剂较粉针剂体外细胞毒效应强。

Objective：To compare the cytotoxicity between cisplatin solution and cisplatin powder in vitro. Methods：Hela, human cell line, was cultured in Dulbecco＇s modified Eagleg medium （DMEM） containing 10% Neonatal Bovine Serum with or without various concentrations of cisplatin came from solution or powder . Cell viability was examined by methylthiazolyl tetrazolium （MTT） assay and 50% inhibitive concentration（ IC50 ） of cisplatin was examined. Cytotoxicity of cisplatin solution and cisplatin powder in vitro was compared using Student＇s t - test. Results： Compared with cisplatin powder group, at the same concentration of cisplatin, in cisplatin solution group, cell viability declined and the 50% inhibitory concentration （ IC50 ） of DDP for cisplatin solution was （ 14.01 ± 0.07） μg · ml^-1 and IC50 of DDP for cisplatin powder was （ 15.03 ± 0.26） μg · ml^-1 （ p = 0. 003 ）. Conclusion： Compared with cisplatin powder, cisplatln solution has a higher cytotoxicity effect in vitro.