血清胸腺嘧啶脱氧核苷激酶1在结直肠癌中的表达及其临床意义

The expression and clinical significance of serum TK-1 in colorectal carcinoma

目的:检测结直肠癌患者血清胸腺嘧啶脱氧核苷激酶1(STK1),探讨其早期诊断、复发转移和治疗疗效的预测意义。方法:应用化学增强发光(ECL)点印迹法定量检测20例术前及术后化疗期结直肠癌患者STK1;同期定量检测外周血CEA;15例健康志愿者作为对照组。结果:结直肠癌患者20例,STK1浓度为1.935±2.068pM;健康人群15例,浓度为0.59±0.29pM;P=0.009,结直肠癌患者中明显高于健康人群。结直肠癌全组中STK1阳性率40%(8/20),健康人群中0%(0/10),P=0.005,有统计学意义;STK1灵敏性40%,特异性100%,阳性预测价值100%。结直肠癌术前患者中STK1阳性率66.67%(6/9),与健康人群比较,P=0.001,具有显著统计学差异;与结直肠癌Ⅳ期正在化疗期患者18.18%(2/11)比较,P=0.028,有统计学差异;与术前患者CEA0%(0/9)比较,P=0.031,有统计学差异。20例结直肠癌全组中,CEA阳性率20%(4/20),低于STK1阳性率(40%)。结论:STK1浓度在结直肠癌患者中明显高于健康人群;其阳性率在术前患者中最高,化疗期患者中较低;提示STK1在结直肠癌的诊断、复发转移以及治疗疗效的预测方面可能较CEA更有优势。

Objective：To detect serum TK1 in patienS of colorectal carcinoma, and study its clinical significance. Methods ：The serum TK1 was detected by enhanced chemoluminescence（ECL） dot blot assay method in 20 colorectal carcinoma, in the same term quantitative to detect the serum CEA, and 15 healthy volunteers as contrast. Results： The density of STK 1 in 20 colorectal carcinoma was ：1. 935 ± 2. 068pM ; In 15 healthy indivduals 0.59 ± 0.29pM; P = 0. 009. Form STK1 positive rate 40% in 20 colorectal carcinoma （8/20）, compared with the rate （0%） in healthy persons,P = 0. 005. STK1 40% of sensitivity, 100% of specificity, positive predict value was 100%. STK1 of preoperative patient , positive rate was 66.67% （6/9）, compared with healthy persons, P = 0.001, In patients of colorectal carcinoma Ⅳ stage positive rate was 18.18% （2/11 ）, P = 0. 028 . CEA of preoperative patients was 0% （0/9） ,P =0. 031. In 20 colorectal carcinoma： CEA positive rate was 20% （4/20）, obviously lower than the positive rate of STK1. Conclusion： STK - 1 density in colorectal carcinoma is obviously higher than in healthy person; STK - 1 positive rate is the most highest in preoperative patient, in period of postoperative chemotherapy is low; STK- 1 positive rate is higher than t CEA positive rate in colorectal carcinoma; STK1 may have a clinical meaning even more than CEA in prediction of the diagnosing, recuring and metastasis , the prediction of curative effect.