固始鸡免疫器官内细胞凋亡相关蛋白Bax和Bcl-2的动态表达

Dynamic Expression of the Apoptosis Related Protein Bax and Bcl-2 in Immunity Organs of Gushi Chickens

应用免疫组织化学技术和Leica显微图像处理系统,对细胞凋亡相关蛋白Bax和Bcl-2在固始鸡免疫器官内的动态表达进行了研究。结果:Bax蛋白在不同发育阶段固始鸡免疫器官内均有表达,但表达量不同;Bax蛋白表达于固始鸡免疫器官内淋巴细胞细胞膜、细胞质和细胞核;Bax蛋白表达阳性细胞在固始鸡不同免疫器官内分布位置不同,呈散在或簇团状分布:脾脏内不同部位均有分布,主要在动脉周围淋巴鞘、红髓、淋巴小结边缘、边缘区,很少出现在淋巴小结内;胸腺内主要分布于胸腺小叶的皮质与髓质交界处,其次是胸腺小叶髓质,极少出现于皮质;法氏囊内阳性细胞主要分布于黏膜靠近上皮细胞的固有膜层内、淋巴滤泡间的区域,在淋巴滤泡边缘的少量淋巴细胞也有Bax蛋白表达,淋巴滤泡内极少有阳性细胞。Bcl-2蛋白在固始鸡免疫器官内的表达与Bax相似,但表达量与Bax相比较少。以上结果表明,细胞凋亡相关蛋白Bax和Bcl-2参与了固始鸡免疫器官内淋巴细胞发育分化过程中的凋亡调控,对免疫器官的稳定发挥重要作用。

Study on dynamic expression of the apoptosis related protein Bax and Bcl-2 in immunity organs of Gushi chickens was performed and analyzed by using immunohistochemistry technology and Leica Microsystems. The results showed that the expressions of Bax protein in different immunity organs were observed, however, numbers of the positive cells of Bax protein were different on different developmental stages. The positive Bax-protein was found in cell membrane, cytoplasm and nucleus of lymphocytes. The positive cells were dispersedly （or in clusters） distributed in different places for different immunity organs. Most of the positive cells, in spleen, were found in periarterial lymphatic sheath, red pulp, edge of splenic nodules and marginal zone, few of them in inner of splenic nodules. The positive cells, in thymus, were localized mainly in the connective zone of thymic cortex and thymic medulla , thymic cortex. In Bursa, the majority of positive cells secondly in thymic medulla, and a few in were observed in lamina propria of mucous membrane and areas between lymphoid follicles, a few in edge of lymphoid follicles, and few in inner of lymphoid follicles. The expression of Bcl-2 in the immunity organs of Gushi chickens was similar to that of Bax, but the expression level of Bcl-2 was less than that of Bax. All results mentioned above indicated that the apoptosis related protein Bax and Bcl-2 participated in the regulation of apoptosis of thymocytes and lymphocytes, and played the important roles in keeping the immunity organs stable.