北京郊区旅游景点北亚蜱传斑点热分子流行病学调查

A Molecular Epidemiologic Investigation of North Asia Fever in Scenic Spots of Beijing Suburb

用ＰＣＲ／ＲＦＬＰ技术对北京郊区１１个旅游景点的媒介蜱及小哺乳动物进行斑点热群立克次体的检测，并进行了病原体分离和鉴定。结果从北京航空博物馆附近地区收集的刺猬和其体表寄生的中华革蜱中分离出２株斑点热群立克次体，分别命名为ＢＪＨ－９５株和ＢＪ－９５株，经ＳＤＳ－ＰＡＧＥ、Ｗｅｓｔｅｒｎｂｌｏｔ和ＰＣＲ／ＲＦＬＰ技术进行了鉴定认为是西伯利亚立克次体。结果证明了该立克次体在蜱和动物宿主间呈水平传播，并且表明在被调查的旅游景点中除航空博物馆附近地区外，大多数景点基本上是北亚热的安全区。在刺猬中分离出西伯利亚立克次体为国内。

PCR/RFLP technique was used to detect spotted fever group rickettsiae (SFGR) in ticks and small mammals collected in eleven scenic spots of Beijing suburb.We not only detected Rickettsia sibirica in D.sinicus and hedgehog collected nearby the Museum of Aviation, but also isolated two strains of SFGR from them,named as BJ-95 strain and BJH-95 strain respectively. The two strains were identified as R.sibirica by SDS-PAGE,Western blot and PCR/RFLP. The results demonstrated the existence of horizontal transmission of R.sibirica between ticks and small mammals and showed the most scenic spots except the vicinity of Museum of Aviation being investigated were safe to North Asia Fever.This is the first report on the isolation of R.sibirica in hedgehogs.