安徽省丙型肝炎病毒基因型分布

An Investigation on Genotypes of Hepatitis C Virus in Anhui Province

应用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）和限制性片段长度多态性（ＲＦＬＰ）技术分析安徽省丙肝患者丙型肝炎病毒（ＨＣＶ）基因型。结果表明ＨＣＶ基因型有Ⅰ、Ⅱ、Ⅲ、Ⅳ及Ⅱ／Ⅲ混合型，以Ⅱ型感染（７５．６６％）占优势，其次为Ⅲ型（１３．２３％）和Ⅱ／Ⅲ型（６．３５％），Ⅰ、Ⅳ型各有１例（０．５３％）。安徽省北部地区的ＨＣＶⅢ型多于南部，而Ⅱ型北部则少于南部，不同地理区域ＨＣＶ基因型分布差异有显著性（Ｐ＜０．０５）。有、无输血。

To study the distribution of HCV genotypes in Anhui province,anti HCV positive serum samples were collected from 371 hepatitis C patients, 60 patients under haemodialysis and 100 blood donors. HCVRNA was detected by reverse transcription polymerase chain reaction (RT PCR). Based on the results of PCR positivity,HCV genotyping was investigated by restriction fragment length polymorphism. Results showed that among hepatitis C patients 143 (75.66%) were HCV type Ⅱ infection, 25(13.23%) were HCV type Ⅲ and 12(6.35%) were type Ⅱ/Ⅲ co infection. HCV type Ⅰ and Ⅳ infection appeared in one of each (0.53%). HCV genotype could not be determined in 7 patients because of low HCVRNA titers. These results indicated that HCV Ⅱ infection was the dominant genotype in Anhui Province. Geographically, HCV type Ⅱ infection was higher in the southern areas. The patterns of genotype distribution in the southern and northern areas were significant different ( P <0.05). There was no significant difference between HCV genotypes in relation to the history of blood transfusion.