湖北省HIV-1流行毒株的基因序列测定和亚型分析

Subtype and Sequence Analysis of the C2 V3 Region of gp120 Genes among HIV 1 Strains in Hubei Province

１９９６年８月下旬采集２０份湖北省ＨＩＶ１感染者外周血，分离单个核细胞（ＰＢＭＣｓ）提取核酸使用ＰＣＲ进行扩增获得ＨＩＶ１膜蛋白（ｅｎｖ）基因的核酸片段，并对其Ｃ２Ｖ３及邻区３５０～４５０个核苷酸序列进行了测定和分析。研究结果表明，这２０份样品均为Ｂ亚型的ＨＩＶ１毒株序列，彼此间的基因离散率为１．９％。与国际ＡＥ亚型参考序列及部分地区Ｂ亚型代表株序列相比较，湖北的２０个毒株与流行与我国云南德宏、四川和河南等地的Ｂ亚型毒株序列十分接近，均属于泰国Ｂ亚型；它们的基因离散率在１．９％～３．２％的范围内。根据以上数据及其它资料提示，ＨＩＶ１在湖北的流行时间不长，该流行区毒株的传入与流行在云南德宏州的相同亚型ＨＩＶ１毒株密切相关。

Twenty DNA fragments of HIV 1 env gene were amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from HIV 1 sero positive people from Hubei province in the end of 1996.The C2 V3 region (about 450 bp) was sequenced.Sequence analyses showed that all of them were HIV 1 subtypes,B strain,with 1.9% gene divergence innergroup.The 20 subtype B strains,compared with sequences of several reference HIV 1 strains,were closely related to those found in Thailand,Myanma and Yunnan and other provinces in China.The nucleotide sequence divergence between them ranged from 1.9% to 3.2%. These results suggested that recent epidemic of B subtype HIV 1 strains in Hubei province and the responsible virus were very close to those identified in Yunnan province.