聚合酶链反应检测人胃螺杆菌

Using Polymerase Chain Reaction to Detect Helicobacter heilmannii in Gastric Biopsy Materials

人胃螺杆菌（Ｈｈ）是慢性胃炎的又一病原菌，体外尚不能培养。作者用对应于细菌１６ＳｒＲＮＡ的螺杆菌属特异引物和细菌通用引物配对，建立了一套ＰＣＲ直接检测人胃粘膜活检标本中Ｈｈ的方法。１２例形态学检查证实为Ｈｈ感染者，１１例ＰＣＲ结果阳性，而大肠杆菌、空肠弯曲菌、双歧杆菌等８种常见肠道菌均阴性；普通ＰＣＲ灵敏度达０．１ｎｇＤＮＡ水平，巢式ＰＣＲ则可检测出０．０１ｎｇＤＮＡ。

Helicobacter heilmannii is one of the species of Helicobacter other than Helicobacter pylori noticed in human gastric mucosa. In our study, a polymerase chain reaction (PCR) was designed for the detection of Helicobacter heilmannii in gastric biopsy specimens. Two broad range bacterial 16S rDNA primers and one Helicobacter genus specific 16S rDNA primer were matched for detecting Helicobacter genus bacteria. A Hp specific primer pair was used simultaneously to exclude the presence of Hp. The PCR assay detected 11 out of 12 Hh strains which were confirmed by smears,but did not amplify DNA extracts from some other enteric bacteria such as E.coli, C.jejuni, bifidobacteria. Serial dilution experiments revealed the detection of as little as 0.1pg DNA by PCR and 0.01pg by nested PCR. Our data showed that PCR is a rapid, accurate and sensitive method for the detection of Hh and is thought valuable in the epidemiological investigation of Helicobacter and follow up studies after treatment.