依伯硒啉对培养大鼠皮层神经元缺氧损伤的拮抗作用(英文)

Protection of ebselen against anoxic damage of cultured neurons of cerebral cortex

目的:研究依伯硒啉对神经细胞缺氧损伤的拮抗作用.方法:培养新生大鼠皮层神经元置于95％N_2+5％CO_2.测定了神经元乳酸脱氢酶(LDH)释放,硫代巴比妥酸反应活性物质(TBARS)含量和谷胱甘肽过氧化物酶(GSH-Px)活性.结果:缺氧后,LDH释放和TBARS含量增加;同时,GSH-Px随缺氧时间延长活性下降;依伯硒啉减少缺氧时LDH释放和TBARS的增加,使缺氧神经元的总GSH-Px活性上升.结论:依伯硒啉对神经元的缺氧损伤具拮抗作用.

AIM: To study the protective effect of ebselen on anoxic damage of brain cells. METHODS: On d 10 after plating of the cortical neurons from 1-d-old rat, cultures were placed under 95 % N2 + 5 % CO2 for 2 - 6 h. Lactate dehydrogenase ( LDH) in supernatant, thiobarbituric acid reactive substance (TBARS) and glutathione peroxidase (GSH-Px) activity of neurons were determined. RESULTS: Under anoxia, efflux of LDH and TBARS from cultured neurons increased while GSH-Px activity decreased. Ebselen reduced the efflux of LDH and TBARS in a dose-related manner and increased the total GSH-Px activity. CONCLUSION: Ebselen can protect neurons from anoxic damage.