摘要
目的:研究雷公藤红素(Tri)对胶原性关节炎的作用.方法:足肿测量仪测定足肿变化;酶联免疫吸附法测定血清抗胶原Ⅱ型抗体;皮肤迟发型变态反应水平测定;白细胞介素-2和白细胞介素-1的测定采用[~3H]TdR掺入法.对后足趾—跖关节作病理切片.结果:Tri 15和30 mg·kg^(-1)·d^(-1),发病3天后给药,改善足肿,抑制抗胶原Ⅱ型抗体水平和迟发型变态反应水平;抑制白细胞介素.1和白细胞介素-2产生;病理检查显示Tri明显抑制关节炎大鼠病理改变.结论:Tri对胶原性关节炎有治疗作用.
AIM: To study the therapeutic effect of tripterine ( Tri ) on collagen-induced arthritis ( CIA ). METHODS: Collagen type Ⅱ (Col) 1.5 mg was injected intradermally to induce CIA in rats. Hind paw volumes of rats were measured with a water displacement method. The serum anti-collagen antibody was measured by an enzyme-linked immunosorbent assay. Delayed hypersensitivity was reflected by skin response to Col. Interleukin-1 (IL-1) and interleukin-2 (IL-2) activities were evaluated by [3H]TdR uptake. Joint was evaluated histologically. RESULTS: Tri 15 and 30 mg·kg-1·d-1 given ig to rats 3 d after the first sign of arthritis reduced inflammatory swelling, suppressed humoral and skin response to Col, inhibited IL-2 and IL-1 production, reduced pathological progression of joint. CONCLUSION: Tri has a therapeutic effect on CIA.
出处
《中国药理学报》
CSCD
1997年第3期273-273,共1页
Acta Pharmacologica Sinica
关键词
胶原
关节炎
雷公藤红素
collagen
arthritis
tripterine
antibodies
delayed hypersensitivity
interleukin-1
interleukin-2
