抗核心脂域McAb对LPS体外诱导细胞释放IL-1及其mRNA表达的影响

EFFECTS OF MONOCLONAL ANTIBODIES TO CORE GLYCOLIPID REGION OF LIPOPOLYSACCHARIDES ON PRODUCTION AND mRNA EXPRESSION OF INTERLEUKIN 1 IN VITRO

采用细胞因子活性检测，Ｎｏｒｔｈｅｒｎｂｌｏｔ及ＲＴ－ＰＣＲ等方法，在体外研究了抗细菌核心糖脂域ＭｃＡｂ（ＥＬ１、ＥＬ３和３Ｈ４）对ＬＰＳ诱导人外周血单核细胞（ｈＰＢＭＣ）释放ＩＬ－１及其ｍＲ－ＮＡ表达水平的影响。结果表明，ＥＬ１、ＥＬ３和３Ｈ４在体外有抑制ＬＰＳ诱导细胞释放ＩＬ－１的作用；Ｎｏｒｔｈｅｒｎｂｌｏｔ及ＲＴ－ＰＣＲ结果证实，这３株ＭｃＡｂ能降低ＩＬ－１ｍＲＮＡ表达的水平；提示抗细菌核心糖脂域ＭｃＡｂ可通过与ＬＰＳ的结合而中和ＬＰＳ作用于效应细胞，从而降低或阻碍了效应细胞中ＩＬ－１ｍＲＮＡ的表达，达到抑制细胞释放ＩＬ－１的作用。

The effects of monoclonal antibodies(McAb) to core glycolipid(CGL)region of lipopolysaccharides(LPS) on the dynamic change and mRNA expression of Interleukin 1(IL 1)in supernatants of cultured human peripheral blood monocyte(hPBMC) induced by LPS in vitro were studied by cytokine bioassay,Northern blot and RT PCR analyses.The results showed that the IL 1 levels in supernatants of hPBMC were reduced in groups treated with anti CGL McAbs (EL1,EL3 and 3H4).Northern blot and RT PCR analyses showed that mRNA expression of IL 1 in hPBMC after LPS induced were also inhibited by these three anti CGL McAbs.These evidences suggest that anti CGL McAbs are able to influence mRNA expressions of LPS induced cytokines by blockade of the combination of LPS with effector cell,and consequently reduce its production.