肝细胞生长因子对肺癌细胞运动和侵袭的影响及原癌基因C-met的表达

REGULATION OF MOTILITY AND INVASIVENESS OF LUNG CANCER CELLS BY HEPATOCYTE GROWTH FACTOR AND EXPRESSION OF PROTO ONCOGENE C met

肝细胞生长因子（ＨＧＦ）可调节许多细胞的运动和生长，检测了ＨＧＦ对５种人类非小细胞肺癌细胞运动和侵袭的作用，有３种细胞的运动和侵袭力在ＨＧＦ刺激后增加。用ＲＮＡ印迹检测ＨＧＦ受体——原癌基因Ｃ－ｍｅｔ产物，可证实这几种细胞均有Ｃ－ｍｅｔｍＲＮＡ存在。细胞蛋白印迹检测进一步证实了ＨＧＦ受体的存在。这些结果表明ＨＧＦ可能在人类非小细胞肺癌的侵袭和转移方面扮演重要角色。

Hepatocyte growth factor((HGF),also known as Scatter factor(SF),regulates both the motility and growth of a number of cell types.Had determined the effects of HGF/SF on the motility and invasiveness of 5 human non small cell lung cancer cell lines(NSCLC).The motility and invasiveness of 3 of these cell lines were increased by HGF/SF.The effect was completely blocked by an anti HGF/SF antibody.Northern blotting studies demonstrated presence of message for the HGF/SF receptor C met in all cell types.Presence of the receptor protein was further confirmed with Western blotting studies.These results suggest that HGF/SF may contribute an important role in motility,spreading and invasion of human lung cancer cell lines.