枸杞多糖对小鼠淋巴细胞信号系统的效应

EFFECT OF LYCIUM BARBARUM POLYSACCHARIDE(LBP) ON THE LYMPHOCYTE SIGNAL TRANSDUCTION SYSTEM IN MICE

对枸杞多糖（ＬＢＰ）发挥免疫调节效应的信号传导系统进行探讨。结果显示，５０～４００μｇ／ｍｌＬＢＰ可剂量依赖性的升高小鼠淋巴细胞内ｃＡＭＰ和ｃＧＭＰ的水平，５０μｇ／ｍｌ尚可升高ＰＭＡ活化的脾淋巴细胞内ｃＧＭＰ的水平。另外，１００μｇ／ｍｌＬＢＰ可增加ＣｏｎＡ活化后的小鼠脾淋巴细胞膜上的ＰＫＣ活性。说明ＬＢＰ的作用途径可能是通过影响ｃＡＭＰ／ｃＧＭＰ系统以及促进ＰＫＣ活性来发挥免疫调节效应的。

Had investigated the immunoregulatory of LBP on lymphocyte signal transduction system in mice. The results showed that 50～400 μg/ml LBP increased both the cellular cAMP and cGMP level in dose dependent manner, and 50 μg/ml LBP also increased the cGMP level of the lymphocytes activated by PMA. Alternately, 100 μg/ml LBP could enhance the membrane PKC activity of lymphocytes activited by ConA. The results suggested that the action way of LBP is made by cAMP/cGMP system and PKC activity to play immunoregulatory effects.