摘要
对河南洛阳地区汉族进行了HLA-DRB1*04等位基因的PCR-SSO分型,采用第12届国际组织相容性专题讨论会(12thIHWC)推荐的引物和序列特异性寡核苷酸探针,可检测17个DRB1*04组特异性等位基因(DRB1*0401-0417)。在116份无血缘关系健康人DNA标本中有16份为DRB1*04,其中1份为纯合,DRB1*04的基因频率为0.0715。DRB1*04等位基因分布于5个不同的型别,分别是DRB1*0401、0403、0404、0405和0406。基因频率以DRB1*0405最高(0.0262),DRB1*0406其次(0.0174),二者分别构成DRB1*04基因组的35.8%和23.8%。其它3种等位基因的基因频率均较低(0.0087)。此外对DRB1*04等位基因在中国南北方汉族、民族(日本,中国)及人种(白种,黄种)中的分布进行了比较与讨论。将为HLA与疾病相关性、移植免疫及人类学研究提供有价值的参考数据。
The HLA DRB1 *04 alleles of Chinese han nationality from Luoyang area in Henan province have been typed by the polymerase chain reaction(PCR) and sequence specific oligonucleotide probes(SSO) which were recommended by the 12th international histocompatibility workshop and conference(12 th IHWC).Seventeen distinct group specific types of DRB1 *04 alleles(DRB1 *0401 to 0417) could be detected with the probes.In 116 unrelated individuals there were 16 samples in which one homozygote included were DRB1 *04.The gene frequency of DRB1 *04 was 0.071 5.The allele distribution of DRB1 *04 were identified in five different patterns which were DRB1 *0401,0403,0404,0405 and 0406.The gene frequency distribution of DRB1 *04 alleles demonstrated as DRB1 *0405 with the highest gene frequency(0.026 2) and DRB1 *0406 with the second(0.017 4).The gene frequencies of the two alleles occupied 35.8% and 23.8% of total respectively.The other three allele frequencies were rare(0.008 6).The differences of DRB1 *04 gene frequency distribution between Southeern and Northern Chinese han nationality,national groups(Japanese,Chinese) and races(Caucasoid,Mongoloid)were compared and discussed.The data would provide a valuable reference for researching of HLA disease association,transplantation immunology and anthropology.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1997年第5期300-302,共3页
Chinese Journal of Immunology
基金
全军医药卫生科研基金