高效液相色谱法测定阿司匹林肠溶片中游离水杨酸的含量

Determination of Salicylic Acid in Enteric-Coated Aspirin Tablets by HPLC

目的：建立高效液相色谱法测定阿司匹林肠溶片中游离水杨酸含量的方法。方法：HPLC法，色谱柱：Diamonsil C18（4．6mm×250mm，5μm）；流动相：乙腈．甲醇旬-0.01mol·L^-1磷酸二氢钾-三乙胺（10：50：40：0．1）用磷酸调节pH=3．4；检测波长：304nm；柱温40℃；流速：1mL·min^-1。结果：水杨酸农度在0．7665-61．32mg·L^-1范围内与其峰面积成良好的线性关系，回归方程：Y=5027．8＋29661X。r=0．9999。高中低3个质量浓度的回收率平均值为100．4％（n=9），RSD=1．6％。结论：本法灵敏度高，重现性好，结果准确。

Objective：To establish a method for determination of Salicylic Acid in Enteric-Coated Aspirin Tablets by RP-HPLC. Methods：HPLC,the analytical column was Diamonsil C18（4. 6 mm ×250 mm,5 μm） ;The mobile phase was acetonitrile-methanol-0. 01mol · L^-1potassium phosphate monobasic-triethylamine （ 10： 50： 40： 0. 1 ） ,adjusted to pH 3.4 with phosphoric acid,The detected wavelength was 304 nm and column temperature was 40 ℃, flow rate was 1 mL · min^-1. Results： The standard curve was linear in the range from 0. 766 5 to 61.32 mg·L^-1 and thecorrelation coefficient was 0. 999 9. The average recovery was 100. 4% （n =9）. The LOD was 0. 3 mg·L^-1. Concluslon;The proposed method is sensitive, precise and accurate.