摘要
目的了解接种冻干甲型肝炎（甲肝）减毒活疫苗[Hepatitis A（Live）Vaccine，Freezedried；Hep A]后，机体能否产生特异性细胞免疫应答。方法研究对象经HepA（H2株）1剂次免疫，流式法检测淋巴细胞亚群CD2^＋、CD4^＋、CD8^＋的百分率及表达干扰素（IFN）-γ及白细胞介素（IL）-4的阳性细胞百分率；T淋巴细胞的特异性增殖试验（MTT法）检测T淋巴细胞增殖反应；酶联免疫斑点法（ELISPOT）检测淋巴细胞的IFNγ分泌能力。结果接种后2周，CD^＋上升，以4周上升为显著，与接种前相比，差异有显著的统计学意义（t=2．571，P〈0．05）；接种后4周～3个月，淋巴细胞对特异性刺激物甲肝病毒的反应都有明显增强，与接种前相比，差异均有非常显著的统计学意义（t=3．215～3．959，P均〈0．01）；接种后2周，表达IL-4和IFN-γ的阳性细胞百分率上升，以IL-4为明显，与接种前相比，差异有显著的统计学意义（t=2．197，P〈0．05）；接种后3年，用ELISPOT法检测IFN-γ，阳性率为57．1％（8／14）。结论接种HepA（H2株）后，机体能有效地产生特异性细朐免疫席答。
Objective To study the specific cellular immune responses of humans induced by Freeze-dried Live Attenuated Hepatitis A Vaccine（H2 Strain）. Methods Sixteen subjects,who had undetected anti-HAV antibody, were inoculated with one dose Freeze-dried Live Attenuated Hepatitis A Vaccine（H2 Strain）. T lymphocyte sub-cluster CD3^＋ , CDf , CD8^＋ and the expressing cells of IFN-γ,IL-4 were tested by FCM. Lymphoproliferative responses were measured by MTT assay. IFN-γ-producing cells were detected by ELISPOT assay. Results Compared with prevaccination,the number of CD4^＋ increased at two weeks after vaccination,then reached peak level at four weeks （t=2. 571 ,P 〈 0. 05）; Lymphoproliferative responses to HAV was obviously increased at 4 weeks to 3 months （t =3. 215 ～ 3. 959,P 〈 0.01） ; The levels of ceils of both IFN- γ, and IL-4 increased at two weeks after vaccination,which the increasing of IL-4-expressing cells was higher than that of IFN-γ cells. The difference has obvious statistic signification （t=2. 197, P〈0.05）. At 3 years after vaccination, posttive of IFN-γ-producing cells were 57.1% by ELISPOT assay. Conclusion The can elicit Specific cellular immune responses can be produced after administrating Freeze -dried Live Attenuated Hepatitis A Vaccine （H2 Strain）.
出处
《中国疫苗和免疫》
CAS
2008年第3期246-249,共4页
Chinese Journal of Vaccines and Immunization
