高效液相色谱-紫外法检测尿中8-羟基脱氧鸟苷

Determination of 8-Hydroxy-2-deoxyguanosine in human urine by high-performance liquid chromatography

目的：观察利用高效液相色谱-紫外法检测尿液8-羟基脱氧鸟苷（8-OHdG）的效果。方法：尿液经前处理后,利用美国Agilent高效液相色谱仪检测8-OH-dG水平。结果：8-OHdG峰与杂质峰分离良好,8-OHdG浓度在0.5～20μg/ml之间呈良好线性,流动相最佳的pH为3.5,回收率在98.6%～100.1%之间,批内差异（CV）均低于8%。结论：高效液相色谱-紫外法是一种灵敏度较高、重复性好、易推广的尿液8-OHdG简便检测方法,值得推广。

Objective：To observe results of detecting 8 - hydroxydeoxyguanosine （8 - OHdG） in urine by high - performance liquid chromatography. Methods： To detect 8 - OhdG level with the United States Agilent HPLC after dealing with urine. Results：The separation of 8 - OhdG＇s peak from impurities＇ was good, 8 - OhdG＇s concentration between 0. 5 μg/ml and 20 μg/ml was linear with the best mobile pH at 3.5, the recovery rate varied from 98.6% to 100. 1%. The difference （CV） was lower than 8%. Conclusion： HPLC is a highly sensitive, reproducible and easy method to promote detection method of the urine 8 - OHdG.