高效液相色谱法测定保健食品中的番茄红素

HPLC determination of lycopene in microcapsules in health foods

目的：采用高效液相色谱法测定保健食品中的番茄红素（胶囊或片剂）。方法：样品用水溶解破膜,以乙酸乙酯提取,用HPLC检测番茄红素的含量。结果：本方法线性范围为0.0～10.0μg/ml,最低检出浓度为0.1μg/ml,加标回收率为98.36%～104.8%,相对标准偏差为2.3%～4.2%。结论：本方法操作简单,灵敏度高,线性范围宽,不失为一种较好的测定方法。

Objective： A HPLC method for determination of lycopene in health foods （in capsules or tablets ）was established. Methods ：The sample was dissolved by pure -water to break its film, then extracted by ethyl acetate. The content of lycopene was detected by HPLC. Results：The linear range of lycopene was 0 - 10.0 μg/ml, r = 0. 9998, the average recovery was from 98. 36% to 104. 8% with RSD less than 4. 2%. The detection limit of lycopene was 0. 1 μg/ml. Conclusion： Using this method to determine lycopene in health foods is simple, sensitive and of brand linear range.