HPLC法测定蜂王浆及蜂王浆冻干粉中的10-羟基-α-癸烯酸

Determination of 10-Hydroxy-α-Decanoic Acid in royal jelly and lyophilized royal jelly by HPLC method

目的：摸索高效液相方法测定10-羟基-α-癸烯酸的检测条件和方法,补充国家标准中检测方法的不足。方法：蜂王浆及其冻干粉中的10-羟基-α-癸烯酸经不同有机相提取后,采用不同流动相经C18色谱柱分离,紫外检测器检测。结果：采用无水乙醇＋0.01 mol/L磷酸=5.5＋4.5的10 ml混合液作为提取液,经超声处理,放置冰霜过夜沉淀取上清液试验,用甲醇＋0.01 mol/L磷酸溶液=55＋45作为流动相,检出限为1.6 mg/kg,在0～0.2 mg/ml范围内,线性关系良好,r=0.9996,RSD〈2%,加标回收率98%～101%。结论：该方法前处理简单、结果稳定、操作方便,符合大多数国家标准的定量习惯,可用于蜂王浆及蜂王浆冻干粉中的10-羟基-α-癸烯酸的测定。

Objective：To explore the detective condiction and approach of 10 -hydroxy-α-decanoic acid with HLPC to replenish the deficient in government standard. Methods： 10 - hydroxy - α - decanoic acid in royal jelly and lyophilized royal jelly are extracted with various organic phase, then isolated via C18 chromato collumn by diverse mobile phase, detected by UV detector. Results：The mixture of 5.5 ml dehydrated alcohol and 4. 5 ml 0.01 mol/L H3 PO4 were used for mobile phase uhrasounding. Overnight in refrig. Precipitate the supernatant were for detection. Use the methyl alcohol ＋ 0. 01 mol/L phosphoric acids aqua =55 ＋45 as mobile phase. The detection limit was 1.6 mg/kg. The linear equation was good between 0 -0. 2 mg/ml,r = 0. 9996,RSD 〈 2%. The recover was 98 % - 101%. Conclusion： The pre - processing of the approach is simple, and consequence is stable and convenient to manipulate. Consistent with majority goverment standard hence it can be used to the detection of 10 - hydroxy - α - decanoic acid in royal jelly and lyophilized royal jelly.