微波消解-电感耦合等离子体质谱测定全血中钙铁锌铜锰硒钼铅

Determination of Ca,Fe,Zn,Cu,Mn,Se,Mo and Pb in adult whole blood by microwave digested-inductively coupled plasma mass spectrometry

目的：建立微波消解-电感耦合等离子体质谱（ICP-MS）测定全血中钙（Ca）、铁（Fe）、锌（Zn）、铜（Cu）、锰（Mn）、硒（Se）、钼（Mo）、铅（Pb）的分析方法。方法：采用微波消解技术对血样进行消解处理后,定容到10 ml容量瓶中,用ICP-MS进行分析。结果：整个方法的检出限可以满足测定的要求,精密度优于2.2%,回收率在95.0%～105.0%之间。对标准参考物质的分析结果令人满意。结论：可用微波消解-电感耦合等离子体质谱法测定全血中Ca、Fe、Zn、Cu、Mn、Se、Mo和Pb,该方法简单、快速和准确,适于人全血样中微量元素的测定。

Objective：To develop a method for direct determination of Ca,Fe,Zn,Cu, Mn, Se,Mo and Pb in adult whole blood by microwave digested- inductively coup led plasma mass spectrometry. Methods：Trace elements in whole blood digested by microwave were detected by inductively coupled plasma mass spectrometry. Results：The detection of limit was good, the RSD less than 2. 2%, recoveries 95.0% - 105. 0% and the results of analyzing standard materiel were satisfactory. Conclusion： This method is simple, sensitive and accurate and can be used to determine Ca, Fe,Zn, Cu, Mn, Se, Mo and Pb in adult whole blood.