RP—HPLC测定不同产地盾叶薯蓣中3种甾体皂苷的含量

RP-HPLC determination of three steroidal saponins in Dioscorea zingiberensis from different habitate

目的:建立反相高效液相色谱法测定鲜盾叶薯蓣根茎中盾叶皂甘 G(zingiberenin G)、盾叶皂苷 A(zingiberenin A)和纤细皂苷(gracillin)的含量。方法:采用 Phenoxent Luna C_(18)(2)色谱住(5μm,250 mm×4.6mm),以乙腈-水为流动相梯度洗脱[0～10min,乙腈从25%→35%;10～15min,乙腈为35%;15～25 min,乙腈从35%→55%;25～35min,乙腈为55%],流速1.0 mL·min^(-1),拄温30℃,检测波长206 nm。结果:样品中各待测组分均能良好分离。盾叶皂苷 G、盾叶皂苷 A 和纤细皂苷的线性范围均为1.0～12.5μg(相关系数为0.9998～0.9999)。平均回收率(n=6)分别为98.1%,99.4%,98.7%;RSD 分别为1.0%,1.8%,0.7%。结论:本方法简便易行、准确可靠,可用于盾叶薯蓣中皂苷的测定和质量控制。

Objective ： To develop an RP - HPLC method for determination of the contents of zingiberenin G, zingiberenin A and gracillin in the fresh rhizomes of Dioscorea zingiberensis C. H. Wright. Methods ： The Phenoxent Luna C18 （2） （5 μm,250 mm ×4.6 mm） was used at 30 ℃ with gradient elution of acetonitrile -water as mobile phase[0 - 10 min, acetonitrile ：25 %→35 % ; 10 - 15 min, acetonitrile ：35 % ; 15 - 25 min, acetonitrile ：35 % →55 % ;25 - 35 min,acetonitrile ：55% ]. The flow rate was 1.0 mL·min^-1. The detection wavelength was set at 206 nm. Results： All saponins of the sample to be determined can be separated obviously. The linear range of calibration curve was 1.0 - 12. 5 μg（r =0. 9998 -0. 9999）. The average recoveries（n =6） were 98. 1% ,99.4% and 98.7% with RSD of 1.0% , 1.8% and 0. 7% , respectively. Conclusion ： This method is simple, accurate, reproducible, and suitable for determination of saponins and quality control of Dioscorea zingiberensis C. H. Wright.