高效液相色谱法同时分离测定氧化苦参碱、槐定碱、槐胺碱、苦参碱、槐果碱

HPLC simultaneous separation and determination of sophoridine,matrine,oxymatrine,sophoramine,sophocarpine

目的:建立高效液相色谱法同时分离测定氧化苦参碱、槐定碱、槐胺碱、苦参碱、槐果碱5种生物碱。方法:色谱柱为Waters XTerra^(TM)RP C_(18)(3.9 mm×150 mm,5 μm),流动相为0.01 mol·L^(-1)磷酸盐缓冲液(pH 8.5)-甲醇(78:22),流速1.0mL·min^(-1),检测波长为220 nm。结果:5种生物碱在上述包谱条件下达到完全的基线分离。氧化苦参碱在2 1～207.0 μg·mL^(-1)(r=0.9999)、槐定碱在10.2～204.1 μg·mL^(-1)(r=0.9999)、槐胺碱在10.1～201.3 μg·mL^(-1)(r=0.9999)、苦参碱在5.0～201.8 μg·mL^(-1)(r=0.9999)、槐果碱在5.0～201.5 μg·mL^(-1)(r=0 9999)的浓度范围内线性良好。结论:本方法简便易行,可用于含该类生物碱的生药与制剂的分析。

Objective：To establish an HPLC method for the simultaneous determination of sophoridine, inatrine, oxyinatrine, sophorainine, sophocarpine. Method： Determination research was taken with ODS column （ 3.9 mm × 150 mm,5 μm）. And the mobile phase consisted of 0. 01 mol ·L^-1 phosphate buffer solution（ pH 8.5 ） -methanol （78： 22）. The flow rate was 1.0 mL·min^-1. UV detection wavelength was 220 nin. Results：A baseline separation was achieved for five alkaloids in determination. The established method showed good linearity. The linear ranges of were 2. 1 - 207.0 μg·mL^-1 （ r = 0. 9999 ） for oxymatrine , 10. 2 - 204. 1 μg·mL^-1 （ r = 0. 9999 ） for sophoridine, 10. 0 - 201.3 μg·mL^-1 （ r =0. 9999 ） for sophorainine, 5.0 - 201.8 μg·mL^- 1 （ r =0. 9999 ） for inatrine, 5.0 - 201.5 μg·mL^-1 （r =0. 9999 ） for sophocarpine. Conclusion ：The method appears easily to operate and suitable for the analysis of medicinal herbs and preparation which contains these alkaloids.