高效液相色谱法测定丁香中丁香酚的含量

HPLC determination of the content of eugenol in Flos Caryophylli

目的:建立 HPLC 法测定丁香中丁香酚的含量。方法:采用 Hypersil C_(18)色谱柱(5 μm,4.6 mm×200 mm),以甲醇-水(65:35)为流动相,流速1.0 mL·min^(-1),紫外检测器于280 nm 测定。结果:丁香酚浓度在14.4～129.6 μg·mL^(-1)范围内线性关系良好(r=0.9999),供试品溶液存24 h 内稳定,平均回收率(n=9)为100.0%。结论:本法简便、快速,结果准确、可靠,重复性好。

Objective：To determine the content of eugenol in Flos Caryophylli by HPLC. Methods：The Hypersil C18 （5μm,4. 6 mm× 200 mm） column was used with mobile phase constituted of methanol- water （65： 35）with a flow rate of 1.0 mL· min^-1 and the detection wavelength was 280 nm. Results. The linear response ranges was 14. 4 - 129.6 μg · mL^-1 （ r = 0. 9999） of eugenol. The sample solution was steady within 24 h. The average recovery was 100. 0% （ n = 9）. Conclusions ： This method is simple, rapid, accurate and reliable.