TAT-血红素氧合酶-1融合蛋白对L-02细胞的转导效果

Transduction of TAT-heme oxygenase-1 fusion protein into L-02 cells

目的观察TAT-血红素氧合酶-1（HO-1）融合蛋白对L-02细胞的转导能力、细胞毒性和转导后细胞内HO-1活性，评价其转导效果。方法L-02细胞与FITC标记的TAT-HO-1融合蛋白孵育4h后，荧光显微镜下观察TAT-HO-1融合蛋白转导入L-02细胞的情况。L-02细胞与TAT-HO-1融合蛋白孵育4h后，采用CCK-8方法测定L-02细胞的活力，采用化学法测定HO-1活性。结果与FITC标记的TAT-HO-1融合蛋白孵育后，L-02细胞内均匀分布绿色荧光；与TAT-HO-1融合蛋白孵育后，L-02细胞活力无明显改变，HO-1活性明显升高。结论TAT-HO-1融合蛋白可有效地转导入L-02细胞，可明显提高细胞内HO-1活性，且无细胞毒性。

Objective To investigate the transduction ability, cytotoxicity and intracellular heine oxygenase-1 （HO-1） activity of TAT-HO-1 fusion protein transduced into L-02 cells. Methods L-02 cells were incubated with FITC-labeled TAT-HO-1 fusion protein for 4 h and were then examined with fluorescence microscope for the transduction ability. After 4 h incubation with TAT-HO-1 fusion protein, viability of L-02 cells was detected by CCK-8 assay and HO-1 activity by chemical method. Results After incubation with FITC-labeled TAT：HO-1 fusion protein, green fluorescence could be observed in L-02 cells. The viability of L-02 cells did not change and HO-1 activity was significantly increased after incubation with TAT-HO-1 fusion protein. Conclusion TAT-HO-1 fusion protein can be effectively, transduced into L-02 ceils and HO-1 activity in L-02 cells is increased without cytotoxicity.